INTRODUCTION: Hormonal disturbances are associated with infertility in women, and they can often be treated through lifestyle modifications and dietary supplements. AIM: This study aimed to determine the correlations between infertility and serum levels of anti-Müllerian hormone (AMH), gonadotrophins, and prolactin according to vitamin D supplementation status. MATERIALS AND METHODS: The current study was conducted on 103 women aged 19-37 years (29 healthy women as a control group and 74 women diagnosed with infertility). Serum levels of the following hormones were examined using enzyme-linked immunosorbent assay: prolactin, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and anti-Müllerian hormone (AMH) in all participating women. RESULTS: We found that women with infertility had significantly higher levels of FSH and LH hormones compared to the control group. Interestingly, women who took vitamin D supplements had significantly lower serum levels of AMH compared to untreated women. Additionally, no significant variations were observed in the serum levels of LH and prolactin between untreated infertile women and women taking vitamin D. CONCLUSIONS: Our findings demonstrate a disturbance in reproductive hormonal levels between healthy and infertile women. Among women with infertility, vitamin D supplementation was associated with lower serum AMH levels, while no statistically significant differences were observed in LH or prolactin levels compared with untreated women, possibly due to other factors or health conditions that affect hormone regulation.