In vitro maturation (IVM) of canine cumulus-oocyte complexes (COCs) is currently characterized by oocyte degeneration and low percentage of oocytes reaching the metaphase II stage of meiosis. Hormones are commonly supplemented in the maturation medium during canine COCs IVM; however, their effect on the COCs during IVM is unclear. The objectives of this study were to assess whether follicle-stimulating hormone (FSH) or progesterone (P4) supplemented during IVM of canine COCs prevented oocyte degeneration and stimulated COCs metabolism. COCs were randomly allocated into CON (CON = Control), FSH (FSH = CON supplemented with FSH), P4 (P4= CON supplemented with P4) and FSH/P4 (FSH/P4 = CON supplemented with FSH and P4) groups, and matured at 38.5°C for 72 h in a 5% CO2/90% N2 atmosphere. Chromatin degeneration and COCs metabolism were assessed at 24 h, 48 h and 72 h of culture. The chromatin data was analyzed using binomial logistic regression and the metabolism data was analyzed using repeated measurements analysis of variance with a statistical significance set at P < 0.05. Regardless of the hormonal supplementation, canine oocytes chromatin degenerated during the IVM period. Hormonal supplementation had a mixed effect on canine COCs metabolism. In all treatment groups, mitochondrial metabolism decreased from 24 h to 48 h and remained low at 72 h. Glycolysis in the hormone supplemented groups was unchanged at 24, 48 and 72h. Supplementation of the maturation medium with hormones did not prevent canine oocyte degeneration nor stimulate COCs metabolism during IVM.