BACKGROUNDS: Ultrasonic therapy has harmfully effects on the structure and function of the testis. Various mechanisms are involved in the impact of ultrasound waves on testes tissue. This study aimed to investigate the effects of ultrasound exposure on cat`s testes by monitoring acute phase proteins, oxidative stress indices, histopathology, and epididymal sperm evaluation over a period of 60 days. Ten adult domestic short hair (DSH) male cats were divided into two equal groups: one exposed to ultrasound and one control groups. The testes were exposed to ultrasonic therapy using 1 MHz, 1.5 W/cm2, three times with a 48-hour interval. Blood samples were taken before treatment and at days 1, 3, and 6 after treatment. Histopathology of the testes and epididymal sperm evaluation were performed at day 60. RESULTS: The results showed that acute phase proteins, serum amyloid (SAA), C-reactive protein (CRP), and alpha-1 glycoprotein (AGP), significantly increased in the first three days after treatment and decreased significantly by day 6 in the treatment group. The increase in acute phase proteins was significantly higher in the treatment group compared to the control group. Malondialdehyde (MDA) and total antioxidant capacity (TAC) did not show significant change in the first week after treatment in the ultrasound exposure group compared to the control group. Testosterone concentration was significantly decreased in the ultrasound exposure group compared to the control group. The epididymal sperm and histopathology evaluation indicated no harmful effect of ultrasound in the treatment group compared to the control group. CONCLUSIONS: Ultrasound exposure of cat`s testes induced an acute phase inflammatory response and decreased testosterone concentration in the first week after treatment, but it did not have harmful effect on testis tissue and epididymal sperm quality.