{"id":69,"title":"Agatharesinol and sequirin C supplementation during in vitro maturation improves porcine oocyte maturation and is associated with improved subsequent embryonic development","title_zh":"在猪卵母细胞体外成熟过程中补充Agatharesinol与sequirin C可改善卵母细胞成熟及后续胚胎发育","url":"https://pubmed.ncbi.nlm.nih.gov/42983770","doi":"10.1292/jvms.26-0340","pmid":"42983770","journal":"J Vet Med Sci","kind":"paper","lang":"en","published_at":"2026-11-04T00:00:00+00:00","fetched_at":"2026-11-08T03:43:52+00:00","authors":["Yamamura M","Hirata M","Namula Z","Nakai A","Hishiyama S","Nagahara M","Tanihara F"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":11.0,"hits":["cumulus","in vitro maturation","ivm","oocyte","oocyte maturation"]},{"slug":"early-embryo","score":1.0,"hits":["blastocyst"]},{"slug":"art-clinical","score":1.0,"hits":["in vitro fertilization"]},{"slug":"embryology-lab","score":1.0,"hits":["embryo culture"]}],"topic_score":11.0,"preference_score":0.0,"preference_labels":[],"llm_score":72.0,"final_score":72.0,"score_dims":{"novelty":75,"rigor":65,"impact":62,"relevance":85,"translation":62},"recommend_reason":"首次将柳杉来源去甲木脂素AR与SC用于猪卵母细胞IVM，并观察到成熟、受精与囊胚率的提升，为IVM体系抗氧化添加剂提供了新候选；但属动物模型、样本与统计细节在摘要层面不可见，临床转化尚远。","keywords":["去甲木脂素","Agatharesinol","sequirin C","体外成熟","猪卵母细胞","胚胎发育"],"selected":1,"story_id":105,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"本研究以猪为模型，评估了源自柳杉的两种去甲木脂素类化合物Agatharesinol（AR）与sequirin C（SC）在体外成熟（IVM）中的作用。研究将卵丘-卵母细胞复合体在含不同浓度AR或SC的培养液中成熟，随后进行体外受精与胚胎培养。结果显示，与对照组相比，两种化合物均提高了卵母细胞成熟率、受精率与囊胚形成率；AR与SC的最适浓度分别为0.5 µM和5 µM。作者据此认为，IVM期间补充AR或SC可改善卵母细胞成熟，并与后续胚胎发育改善相关，提示去甲木脂素类化合物有望作为改善体外胚胎生产的新型生物活性物质。","summary":"Agatharesinol (AR) and sequirin C (SC) are representative norlignans derived from Cryptomeria japonica that exhibit antioxidant properties. However, their biological applications in animal systems remain unclear. This study evaluated the effects of AR and SC supplementation during in vitro maturation (IVM) on porcine oocyte maturation, fertilization, and embryonic development in vitro. Cumulus-oocyte complexes were cultured with AR or SC at various concentrations, followed by in vitro fertilization and embryo culture. Both compounds improved maturation, fertilization, and blastocyst formation rates compared with the controls. Optimal concentrations were 0.5 and 5 µM for AR and SC, respectively. Therefore, AR or SC supplementation during IVM improved oocyte maturation and was associated with improved subsequent embryonic development, highlighting the potential of norlignan-type compounds as novel bioactive agents for improving in vitro embryo production.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}