{"id":64,"title":"Adverse effects of a superovulatory regimen of equine chorionic gonadotropin and gonadotropin-releasing hormone on embryo implantation in mice","title_zh":"eCG联合GnRH超促排卵方案通过雌激素介导的子宫内膜容受性受损降低小鼠胚胎着床率","url":"https://pubmed.ncbi.nlm.nih.gov/42984919","doi":"10.30466/vrf.2025.2054637.4679","pmid":"42984919","journal":"Vet Res Forum","kind":"paper","lang":"en","published_at":"2026-08-15T00:00:00+00:00","fetched_at":"2026-11-08T03:43:52+00:00","authors":["Yang N","Han J","Wang X","Li X","Wu X"],"primary_topic":"art-clinical","topics":[{"slug":"art-clinical","score":7.0,"hits":["embryo transfer","gnrh","gonadotropin","implantation rate"]},{"slug":"animal-models","score":4.0,"hits":["equine","mouse embryo"]},{"slug":"repro-endo","score":2.0,"hits":["ovulatory","progesterone"]},{"slug":"early-embryo","score":1.0,"hits":["blastocysts"]},{"slug":"oocyte","score":1.0,"hits":["oocytes"]},{"slug":"endometrium","score":1.0,"hits":["endometrial receptivity"]}],"topic_score":7.0,"preference_score":5.0,"preference_labels":["可转化到 ART 实验室的发现"],"llm_score":72.0,"final_score":77.0,"score_dims":{"novelty":68,"rigor":70,"impact":65,"relevance":82,"translation":70},"recommend_reason":"研究以胚胎移植分离胚胎与母体因素，提示超促排卵激素方案可直接损害子宫内膜容受性，对理解ART促排卵与着床失败机制有参考价值；但证据来自小鼠，外推到人类需谨慎。","keywords":["eCG","GnRH","超促排卵","胚胎着床","子宫内膜容受性","Lif"],"selected":1,"story_id":446,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该研究在小鼠中采用胚胎移植排除胚胎因素，比较对照、eCG、GnRH及eCG+GnRH四组受体的着床情况。结果显示，eCG组和eCG+GnRH组着床率显著低于GnRH组与对照组，其中联合组最低；联合组血清雌激素显著升高、孕酮降低，孕酮/雌激素比值下降，子宫内膜容受性相关Lif基因表达亦显著降低。作者认为，eCG联合GnRH超促排卵可通过雌激素介导的子宫内膜容受性受损降低小鼠胚胎着床率。","summary":"Although equine chorionic gonadotropin (eCG) combined with gonadotropin-releasing hormone (GnRH) is known to improve reproductive rates by increasing the numbers of ovulating oocytes, the actions of these hormones on embryo implantation have not been elucidated. We herein eliminated the effects of embryos by performing embryo transfer and elucidated the effects on embryo implantation after hormonal treatment. The recipient mice were allocated to four groups, including control, eCG, GnRH, and eCG + GnRH groups. Well-developed blastocysts were transferred into recipients, and implantation sites were counted five days after embryo transfer. Our results revealed that embryo implantation rates in the eCG and eCG + GnRH groups were significantly lower than GnRH and control groups; eCG and eCG + GnRH groups didn't differ, and the implantation rate was the lowest in the eCG + GnRH group. When we evaluated estrogen and progesterone concentrations in four groups on embryonic day 0.5 to embryonic day 3.5, we noted that serum estrogen levels in eCG + GnRH group were significantly higher than the other groups, while progesterone levels were significantly lower than the control group, and that the progesterone/estrogen ratio was significantly reduced relative to the control group. Finally, the expression of the endometrial receptivity-related Lif gene was significantly diminished in the eCG + GnRH and eCG groups compared to the other groups. These results suggest that eCG combined with GnRH as a super-ovulatory regimen reduced mouse embryo implantation rates via an estrogen-mediated impairment of endometrial receptivity.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}