{"id":56,"title":"Morphokinetic characterization of human embryos derived from capacitation in vitro maturation versus controlled ovarian stimulation with intracytoplasmic sperm injection: a retrospective cohort study using time-lapse imaging","title_zh":"双相获能体外成熟与控促排卵ICSI来源人胚胎的形态动力学特征：基于延时成像的回顾性队列研究","url":"https://pubmed.ncbi.nlm.nih.gov/42986520","doi":"10.1007/s10815-026-04070-2","pmid":"42986520","journal":"J Assist Reprod Genet","kind":"paper","lang":"en","published_at":"2026-11-05T00:00:00+00:00","fetched_at":"2026-11-08T03:43:52+00:00","authors":["Dang AH","Nguyen TC","Pham TD","Le PT","Lam HT","Nguyen TT","Huynh BG","Ho TM","Vuong LN","Pham HT"],"primary_topic":"art-clinical","topics":[{"slug":"art-clinical","score":11.0,"hits":["assisted reproduction","controlled ovarian","icsi","infertility","intracytoplasmic sperm injection"]},{"slug":"oocyte","score":9.0,"hits":["in vitro maturation","ivm","ovarian"]},{"slug":"male","score":9.0,"hits":["capacitation","male factor","sperm"]},{"slug":"embryology-lab","score":8.0,"hits":["morphokinetic","time-lapse"]},{"slug":"early-embryo","score":2.0,"hits":["blastocyst","compaction"]},{"slug":"pgt","score":1.0,"hits":["preimplantation genetic"]}],"topic_score":11.0,"preference_score":11.0,"preference_labels":["人类胚胎材料","可转化到 ART 实验室的发现"],"llm_score":80.0,"final_score":91.0,"score_dims":{"novelty":72,"rigor":78,"impact":76,"relevance":95,"translation":85},"recommend_reason":"系统比较CAPA-IVM与COS-ICSI囊胚形态动力学，样本量尚可且采用倾向评分匹配，对time-lapse判读和IVM实验室应用有直接参考价值；但为单中心回顾性研究，结论需外验证。","keywords":["CAPA-IVM","time-lapse","形态动力学","囊胚","IVM","辅助生殖"],"selected":1,"story_id":62,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":4,"has_interpretation":false,"journal_key":"jarg","journal_tier":"standard","journal_if":3.0,"is_mock":false,"summary_zh":"回顾性队列纳入232对夫妇876枚胚胎，将CAPA-IVM与COS-ICSI按女性年龄和可分析胚胎数1:1倾向评分匹配，培养至囊胚并以time-lapse比较形态动力学。CAPA-IVM胚胎原核出现更晚、原核消失及t3/t5更早，但致密化和囊胚形成更晚；校正后多数差异仍显著，优质G1/G2比例较低（29% vs 39%）。结论：CAPA-IVM胚胎呈非均一的独特形态动力学模式，延时成像解读需考虑成熟途径。","summary":"PURPOSE: To characterize the morphokinetic profile of blastocyst-stage embryos derived from biphasic capacitation in vitro maturation (CAPA-IVM), using a contemporaneous cohort of controlled ovarian stimulation with intracytoplasmic sperm injection (COS-ICSI) embryos as the reference, with standardized time-lapse imaging. METHODS: This retrospective cohort study was conducted at an assisted reproduction center from March 2021 to December 2024. Eligible women were aged < 40 years and had no prior fertilization failure, gamete donation, planned preimplantation genetic testing, or significant male factor infertility. CAPA-IVM and COS-ICSI cycles were matched 1:1 on a propensity score built from female age and the number of analyzable embryos in the cycle. Embryos were cultured to the blastocyst stage with time-lapse monitoring. Morphokinetic timings were compared using crude, adjusted mixed-effects, and trajectory analyses, with a crude subgroup analysis of good-quality G1/G2 embryos. RESULTS: Overall, 232 couples contributed 876 embryos (421 CAPA-IVM and 455 COS-ICSI). In crude analyses, CAPA-IVM versus COS-ICSI embryos showed later pronuclear appearance (+ 0.71 h; p < 0.001), earlier pronuclear fading (- 0.92 h; p < 0.001), earlier t3 (- 1.42 h; p < 0.001), and earlier t5 (- 1.40 h; p = 0.017), but later compaction (+ 2.00 h; p < 0.01) and blastulation (+ 1.67 h; p < 0.01). Later pronuclear appearance, earlier pronuclear fading, earlier t3, and earlier t5 with CAPA-IVM versus COS-ICSI remained statistically significant after adjustment. There was a lower proportion of good-quality G1/G2 embryos after CAPA-IVM versus COS-ICSI (29% vs. 39%; p < 0.01). In the G1/G2 embryo subset, CAPA-IVM showed later pronuclear appearance but earlier pronuclear fading, t3, and t4. CONCLUSIONS: CAPA-IVM embryos showed a distinct, non-uniform morphokinetic profile, highlighting the need for maturation pathway-specific interpretation of time-lapse data.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}