{"id":499,"title":"Genome-wide CRISPR screening in Spodoptera frugiperda reveals the determinants of insecticide activation and sensitivity","title_zh":"斜纹夜蛾全基因组 CRISPR 筛选揭示杀虫剂活化与敏感性的决定因子","url":"https://doi.org/10.1038/s41467-026-79097-1","doi":"10.1038/s41467-026-79097-1","pmid":null,"journal":"Nature Communications","kind":"paper","lang":"en","published_at":"2026-11-07T00:00:00+00:00","fetched_at":"2026-11-08T03:45:21+00:00","authors":["Huidong Wang","Yueru Ye","Tong Zhang","Bingbing Li","Angela Hayward","Yinjia Wang","Yajuan Xiao","Yajie Kong","Jie Zhong","Cheng Li","Kairan Zuo","Shilong Wang","Ralf Nauen","Yu Shi","Xingliang Wang","Chris Bass","Sanyuan Ma","Shutang Zhou"],"primary_topic":"ethics-policy","topics":[{"slug":"ethics-policy","score":3.0,"hits":["crispr"]}],"topic_score":3.0,"preference_score":0.0,"preference_labels":[],"llm_score":32.0,"final_score":40.0,"score_dims":{"novelty":55,"rigor":50,"impact":18,"relevance":8,"translation":10},"recommend_reason":"内容为农业害虫杀虫剂抗性与毒理机制研究，与生殖医学、辅助生殖及胚胎研究无实质交集，不建议作为本领域日常阅读；仅对关注 CRISPR 筛选方法学的读者有间接参考意义。","keywords":["CRISPR 筛选","草地贪夜蛾","杀虫剂敏感性","功能基因组学","害虫防治"],"selected":0,"story_id":null,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"crossref-cns","source_name":"Crossref · CNS 与大子刊新上线（领域过滤）","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"nat-commun","journal_tier":"cns-sub","journal_if":14.7,"is_mock":false,"summary_zh":"该研究以农业害虫草地贪夜蛾（Spodoptera frugiperda）为对象，利用全基因组 CRISPR 筛选鉴定影响杀虫剂活化与敏感性的关键基因，从功能基因组学层面解析昆虫对杀虫剂的响应机制。原文未提供摘要，具体筛选规模、细胞系或个体水平验证、候选基因数量及分子通路等细节无法确认。该工作属昆虫毒理与农业害虫防治方向，与生殖医学、辅助生殖及胚胎多组学无直接关联，对 ART 实验室与临床决策的参考价值有限；仅其 CRISPR 筛选方法学或可为生殖领域基因功能研究提供间接方法借鉴。","summary":"","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}