{"id":47,"title":"Analysis of sperm processing and cryopreservation procedures in the common marmoset ( Callithrix jacchus) to reduce fragmentation and maximize total and progressive motility","title_zh":"普通狨猴精子处理与冷冻保存流程优化：微流控提升活力并减少DNA损伤","url":"https://pubmed.ncbi.nlm.nih.gov/42990570","doi":"10.64898/2026.10.29.762870","pmid":"42990570","journal":"bioRxiv","kind":"paper","lang":"en","published_at":"2026-10-30T00:00:00+00:00","fetched_at":"2026-11-08T03:43:52+00:00","authors":["Holton RA","Brennan JT","Lipari SI","Pickel J","Denney N","Singh B","Jun J","Zhang Y","Li PP","Robertson CD","Hong I","Huganir R","Kalhor R","Ross CA","Segars JH","Wang X"],"primary_topic":"male","topics":[{"slug":"male","score":6.0,"hits":["dna fragmentation","semen","sperm"]},{"slug":"embryology-lab","score":5.0,"hits":["cryopreservation","thawing"]},{"slug":"art-clinical","score":1.0,"hits":["ivf"]}],"topic_score":6.0,"preference_score":0.0,"preference_labels":[],"llm_score":72.0,"final_score":72.0,"score_dims":{"novelty":70,"rigor":68,"impact":65,"relevance":62,"translation":62},"recommend_reason":"对狨猴基因编辑与IVF实验室的精子处理、冻存标准化有直接实用价值，微流控与冻存方案比较设计较清晰；但属预印本，样本量与统计细节可见信息有限，对人类ART的直接外推价值有限。","keywords":["普通狨猴","精子冷冻保存","微流控","CASA","DNA碎片","辅助生殖"],"selected":1,"story_id":275,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":3,"has_interpretation":false,"journal_key":"biorxiv","journal_tier":"standard","journal_if":null,"is_mock":false,"summary_zh":"该预印本研究以普通狨猴为对象，比较四种孵育前处理及上游法、蔗糖梯度与微流控三种精子处理方式，并用CASA评估精子参数。结果显示38°C TYH培养基孵育30分钟可减少精子聚集，后续微流控处理的总活力与前向运动比例优于上游法和梯度法。在五种冷冻保存方案中，优化方案解冻后稳定达到至少20%前向运动，且未造成DNA损伤。研究为狨猴IVF与基因编辑相关精子处理和冻存提供了标准化参考。","summary":"The marmoset has emaserged as an ideal species for studying complex neurodevelopmental diseases, and IVF is required for gene-editing success. Reliable sperm processing is paramount to several IVF techniques, including traditional insemination. If not used immediately, thermal stress from cryopreservation diminishes sperm quality, requiring improved processing methods. Swim-up and gradient methods may limit adequate sperm quality, and the lack of a standardized freezing protocol often results in impaired post-thaw motility. Thus, reliable standards for sperm processing and cryopreservation in the marmoset are needed to support vital IVF processes. Collected semen underwent one of four pre-processing incubation steps before processing by swim-up, sucrose gradient, or a microfluidics assay. Sperm parameters were assessed using a Hamilton Thorne Computer Assisted Sperm Analysis (CASA) system. To evaluate cryopreservation, microfluidics-processed sperm were subjected to five protocols using different cryoprotectants and cooling methods. After thawing, sperm samples were analyzed with the CASA system and a DNA fragmentation assay. Incubation for 30 minutes at 38°C in TYH media decreased sperm aggregation, and subsequent microfluidics resulted in a higher percentage of motile and progressively motile sperm than swim-up or gradient methods. Our optimized cryopreservation protocol consistently achieved at least 20% progressive motility, a key benchmark for usable marmoset sperm, while previously published or commercial methods were less consistent. The cryopreservation protocol outperformed other published methods, having the least significant change in post-thaw motility without causing DNA damage. These findings establish standardized methods for reliably procuring and storing sperm samples for IVF or husbandry in the common marmoset.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}