{"id":435,"title":"Optimizing the efficiency of assisted reproduction procedures in the common marmoset ( Callithrix jacchus) improved IVF outcomes","title_zh":"优化普通狨猴辅助生殖流程可改善体外受精结局","url":"https://doi.org/10.64898/2026.10.30.763070","doi":"10.64898/2026.10.30.763070","pmid":null,"journal":"bioRxiv","kind":"preprint","lang":"en","published_at":"2026-11-03T00:00:00+00:00","fetched_at":"2026-11-08T03:44:14+00:00","authors":["Holton RA","Brennan JT","Lipari SI","Pickel J","Denney N","Singh B","Winer-Centurelli B","Jun J","Zhang Y","Li PP","Robertson CD","Hong I","Huganir R","Kalhor R","Ross CA","Segars JH","Wang X"],"primary_topic":"art-clinical","topics":[{"slug":"art-clinical","score":10.0,"hits":["assisted reproduction","embryo transfer","gnrh","ivf","ovarian stimulation"]},{"slug":"oocyte","score":2.0,"hits":["oocyte","ovarian"]},{"slug":"embryology-lab","score":1.0,"hits":["cryopreserved"]},{"slug":"repro-endo","score":1.0,"hits":["progesterone"]}],"topic_score":10.0,"preference_score":5.0,"preference_labels":["可转化到 ART 实验室的发现"],"llm_score":66.0,"final_score":71.0,"score_dims":{"novelty":60,"rigor":65,"impact":58,"relevance":68,"translation":62},"recommend_reason":"狨猴是神经发育遗传研究的重要非人灵长类模型，该预印本以2年纵向数据系统梳理孕酮、促排卵时长与取卵次数对IVF/胚胎结局的影响，对非人灵长类ART平台建设有直接参考价值；但样本量与统计细节在可见信息中不充分，且结论向人类ART外推有限。","keywords":["普通狨猴","体外受精","促排卵方案","孕酮","GnRH拮抗剂","卵裂期胚胎"],"selected":1,"story_id":127,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"europepmc-preprints","source_name":"Europe PMC · 预印本（领域内）","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"biorxiv","journal_tier":"standard","journal_if":null,"is_mock":false,"summary_zh":"研究以普通狨猴（Callithrix jacchus）为对象，分析年龄、促排卵周期数与孕酮水平对IVF及胚胎学结局的影响，数据来自2年纵向记录。结果显示：注射氯前列烯醇钠后24小时的孕酮水平、促排卵天数与既往取卵次数均影响周期结局；周期起始血清孕酮较高与受精卵发育至卵裂期概率降低相关；较短促排卵方案（7–14天）联合GnRH拮抗剂与更高受精率相关，并呈较短刺激周期提高卵裂期胚胎比例的倾向。优化刺激方案与胚胎实验室流程后，鲜胚与冻胚移植的种植率均较高。提示较短IVF周期且起始孕酮<10 ng/mL与卵裂期胚胎发育概率升高相关。","summary":"The marmoset has emerged as a model species for genetic studies of complex neurodevelopmental conditions because of their brain development, behavior, communication, and compact size. IVF has been used to generate wild-type and transgenic marmoset embryos, but inefficiency of IVF in the marmoset compared to other primates poses challenges for genetic manipulation. To address this limitation, we analyzed the association between variables of age, number of cycles, and progesterone levels for IVF and embryology outcomes. Longitudinal data collected over 2 years indicated that the progesterone levels 24 hours after Cloprostenol sodium (Estrumate) administration, the number of days of ovarian stimulation during an IVF cycle, and the number of oocyte retrievals that an animal had undergone all influenced IVF cycle outcomes. Higher serum progesterone levels at cycle start were negatively correlated with the likelihood of fertilized eggs progressing to the cleavage stage of embryo development. Additionally, a shorter duration of ovarian stimulation (ranging 7 to 14 days of medication) coupled with a GnRH antagonist correlated with an increased percentage of fertilized eggs. We observed a trend that a shorter hormonal stimulation cycle length was associated with a higher percentage of embryos reaching the cleavage stage. The optimization of stimulation cycle protocol and embryology has led to a high percentage of implantation after embryo transfer regardless of whether the embryo was fresh or cryopreserved. These findings suggest that shorter IVF cycles and a progesterone level <10 ng/mL at cycle start were correlated with an increased likelihood of a cleavage stage embryo development.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}