{"id":410,"title":"Suppression of meiotic double-strand break formation in somatic cells by DREAM factors TCX5/6 maintains genome stability","title_zh":"DREAM 复合体因子 TCX5/6 在体细胞中抑制减数分裂双链断裂形成以维持基因组稳定性","url":"https://pubmed.ncbi.nlm.nih.gov/42886774","doi":"10.1126/sciadv.aef7208","pmid":"42886774","journal":"Sci Adv","kind":"paper","lang":"en","published_at":"2026-10-16T00:00:00+00:00","fetched_at":"2026-11-08T03:44:12+00:00","authors":["Guo Y","Zheng X","Wang T","Li J","Wang C","You C","He XJ","Schnittger A","Qi J","Wang J","Wang Y"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":5.0,"hits":["meiosis","meiotic"]},{"slug":"repro-genetics","score":1.0,"hits":["meiotic recombination"]},{"slug":"ethics-policy","score":1.0,"hits":["regulation"]},{"slug":"omics-methods","score":1.0,"hits":["transcriptomic"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":58.0,"final_score":66.0,"score_dims":{"novelty":76,"rigor":80,"impact":45,"relevance":25,"translation":22},"recommend_reason":"机制新颖、证据链完整（遗传学、转录组和修饰层面相互印证），但研究对象为植物体细胞，与人类生殖与辅助生殖的直接关联较弱，主要作为“减数分裂基因体细胞沉默与基因组稳定性”的交叉参考阅读。","keywords":["DREAM 复合体","TCX5/TCX6","减数分裂基因沉默","DNA 双链断裂","H3K4me3","基因组稳定性"],"selected":1,"story_id":386,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-top-journals","source_name":"PubMed · CNS/大子刊/四大医学刊（生殖相关）","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"sci-adv","journal_tier":"cns-sub","journal_if":11.7,"is_mock":false,"summary_zh":"该研究以减数分裂特异基因 DMC1 的启动子为诱饵，在植物（拟南芥）体系中鉴定出 DREAM 复合体组分 TCX5 与 TCX6，它们可在体细胞中直接抑制 DMC1 等减数分裂基因的转录。转录组分析显示 tcx5/6 突变体叶片中减数分裂基因广泛异位上调；相应体细胞出现 DNA 双链断裂，并对 DNA 损伤超敏。遗传学分析表明，SPO11-1 对异位减数分裂基因激活并非必需，但对突变体体细胞的 DNA 损伤应答必不可少；机制上 TCX5/6 通过 H3K4me3 修饰实现抑制。该工作揭示了体细胞中抑制减数分裂重组、防止基因组不稳定的新机制。","summary":"Meiotic recombination in reproductive cells is initiated by DNA double-strand breaks (DSBs) and requires the expression of meiosis-specific genes, yet how the transcription of those genes is regulated in either meiotic or somatic cells remains unclear. Here, using a representative meiosis-specific DMC1 gene promoter as a bait, we identified two DREAM factors TCX5 and TCX6 required for direct repression of DMC1 in somatic cells. Transcriptomic analyses show broad ectopic up-regulation of meiotic genes in tcx5/6 leaves. Consistently, tcx5/6 somatic cells form DSBs and exhibit hypersensitivity to DNA damage compared to wild type. Further genetic analyses reveal that SPO11-1 is dispensable for ectopic meiotic gene activation but essential for the DNA damage response in tcx5/6 somatic cells. Last, we demonstrated that TCX5/6 repress meiotic gene expression through H3K4me3 modification. This finding uncovers an unexpected mechanism for suppressing meiotic recombination in somatic cells, thus preventing genetic diversity and genome instability in organisms.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}