{"id":377,"title":"Amyloid-mediated RNA uptake by sperm for embryonic delivery","title_zh":"淀粉样纤维介导精液RNA被精子摄取并向胚胎传递","url":"https://pubmed.ncbi.nlm.nih.gov/42933403","doi":"10.1016/j.molcel.2026.10.004","pmid":"42933403","journal":"Mol Cell","kind":"paper","lang":"en","published_at":"2026-10-26T00:00:00+00:00","fetched_at":"2026-11-08T03:44:12+00:00","authors":["Mallen WS","Zabello N","Brenner-Morton S","Sidoli S","Liang FX","Sall J","Yin J","Mathews P","Zhu M","Zhang G","Lee CL","Liao YC","Fitzpatrick AWP"],"primary_topic":"male","topics":[{"slug":"male","score":5.0,"hits":["seminal","sperm"]},{"slug":"art-clinical","score":2.0,"hits":["in vitro fertilization","ivf"]},{"slug":"early-embryo","score":1.0,"hits":["zygote"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":84.0,"final_score":92.0,"score_dims":{"novelty":90,"rigor":82,"impact":82,"relevance":78,"translation":75},"recommend_reason":"首次提出功能性淀粉样纤维作为精液RNA载体、介导RNA经精子进入合子的新机制，多模态成像与IVF功能验证较完整；对精子RNA获得机制、胚胎多组学与ART中RNA递送工具开发均有启发。","keywords":["精液RNA","淀粉样纤维","精子摄取","合子","RNA递送","体外受精"],"selected":1,"story_id":317,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-top-journals","source_name":"PubMed · CNS/大子刊/四大医学刊（生殖相关）","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"mol-cell","journal_tier":"cns-sub","journal_if":14.5,"is_mock":false,"summary_zh":"精液（SF）中的RNA对胚胎发育至关重要，但细胞外RNA如何从精液转移至精子的机制尚不清楚。作者发现精液中功能性淀粉样纤维可结合RNA并将其转运至哺乳动物精子，受精后进一步进入合子。研究显示精液中高阳离子肽与RNA相分离形成淀粉样纤维；冷冻电镜断层成像、体电子显微镜与荧光活细胞成像证实这些纤维被哺乳动物精子内化；生物物理与体外受精实验表明，淀粉样纤维结合的RNA可到达合子，且分子伴侣能调控RNA从纤维上解离。该工作揭示了精液至合子的RNA转运机制，并提出淀粉样纤维作为受精过程中RNA递送工具的潜力。","summary":"Seminal fluid (SF) RNA is essential for embryonic development, but the mechanism by which extracellular RNA is transferred from SF to sperm remains unclear. Here, we identify a role for functional amyloid fibrils in SF that bind and traffic RNA to mammalian sperm and, upon fertilization, to the zygote. We show that highly cationic peptides in SF phase separate with RNA to form amyloid fibrils. Cryo-electron tomography (cryo-ET), volume electron microscopy (volume EM), and fluorescence live-cell imaging reveal that these amyloid fibrils are internalized by mammalian sperm. Furthermore, biophysical and in vitro fertilization (IVF) experiments show that amyloid-bound RNA reaches the zygote, where molecular chaperones can control RNA dissociation from amyloid fibrils. Our work uncovers a mechanism for RNA trafficking from SF to the zygote, establishing amyloid fibrils as potential tools for RNA delivery during fertilization.","interpretation_basis":null,"fulltext_status":"non_oa","interpretation":null,"fulltext":{"available":false,"status":"non_oa","reason":"Europe PMC 未收录免费全文（非 OA，或该来源不在 Europe PMC 全文范围内，如 bioRxiv 预印本）：isOpenAccess=False, inEPMC=False","pmcid":null,"license":null,"chars":0,"source":"Europe PMC","fetched_at":"2026-11-08T03:59:51+00:00"}}