{"id":36,"title":"The making of an egg: Cellular and molecular journeys through human folliculogenesis and oocyte maturation","title_zh":"卵子的形成：人类卵泡发生与卵母细胞成熟的细胞和分子历程","url":"https://pubmed.ncbi.nlm.nih.gov/42994180","doi":"10.1016/j.repbio.2026.101303","pmid":"42994180","journal":"Reprod Biol","kind":"paper","lang":"en","published_at":"2026-11-06T00:00:00+00:00","fetched_at":"2026-11-08T03:43:52+00:00","authors":["Santos T","Calado AM","Saramago J","Sá R","Sousa M"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":13.0,"hits":["follicular","folliculogenesis","in vitro maturation","oocyte","oocyte maturation","oogenesis","primordial follicle"]},{"slug":"art-clinical","score":1.0,"hits":["assisted reproduction"]},{"slug":"embryology-lab","score":1.0,"hits":["cryopreservation"]},{"slug":"fertility-preservation","score":1.0,"hits":["oocyte cryopreservation"]},{"slug":"repro-aging","score":1.0,"hits":["mitochondria"]},{"slug":"ethics-policy","score":1.0,"hits":["regulatory"]},{"slug":"industry","score":1.0,"hits":["acquisition"]}],"topic_score":13.0,"preference_score":5.0,"preference_labels":["可转化到 ART 实验室的发现"],"llm_score":78.0,"final_score":83.0,"score_dims":{"novelty":65,"rigor":68,"impact":76,"relevance":96,"translation":82},"recommend_reason":"综述将卵泡发生的形态学、分子调控与ART临床问题整合，适合关注卵母细胞成熟、IVM和冷冻策略的读者；但属文献归纳，非原始研究，证据强度有限。","keywords":["人卵泡发生","卵母细胞成熟","Hippo通路","PI3K/mTOR","体外成熟","卵母细胞冷冻"],"selected":1,"story_id":40,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"reprod-biol","journal_tier":"standard","journal_if":2.0,"is_mock":false,"summary_zh":"本文为综述，系统更新人类卵泡发生与卵母细胞成熟的形态学和分子学认识，重点梳理Hippo及PI3K/mTOR通路在原始卵泡休眠与激活中的调控作用；结合经典形态标志与定量体视学数据，描绘卵母细胞线粒体、滑面内质网复合体和皮质囊泡在减数分裂I前、中期I和中期II的动态空间重组；并将分子检查点与辅助生殖临床问题相连，讨论对体外成熟、卵母细胞冷冻保存及无创质量评估的转化意义。原文未提供原始实验数据。","summary":"Human folliculogenesis has been extensively reviewed, but gaps between morphology and molecular facets remain to be filled. We here detailed update human folliculogenesis at the morphological and molecular aspects and provided a link to oocyte maturation and competence acquisition, and, as well, to the novel findings on oocyte ultrastructure. While human folliculogenesis and oogenesis set the baseline of female fertility, a major challenge remains to bridge the gap between macro-morphological transitions and limited molecular pathways. Here, we provide a comprehensive review of recent conceptual advances in human follicular development, with a focus on emerging regulatory networks such as the Hippo and PI3K/mTOR pathways that regulate primordial follicle dormancy and activation. We combine classic morphological landmarks with recent quantitative stereological data to map the dynamic spatial reorganization of oocyte organelles (mitochondria, smooth endoplasmic reticulum complexes, and cortical vesicles) during prophase-I, metaphase-I and metaphase-II. Furthermore, we emphasize the translational significance of these cellular dynamics by connecting specific molecular checkpoints to clinical problems encountered in assisted reproduction. Understanding these synchronous nuclear-cytoplasmic maturation dynamics offers us important information to optimize in vitro maturation protocols, refine oocyte cryopreservation strategies and improve non-invasive quality assessments in reproductive medicine.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}