{"id":352,"title":"The CCCH-type zinc-finger PfMD3 promotes translation for malaria parasite transmission","title_zh":"CCCH型锌指蛋白PfMD3通过促进翻译推动疟原虫传播","url":"https://pubmed.ncbi.nlm.nih.gov/42977744","doi":"10.1038/s41467-026-77156-1","pmid":"42977744","journal":"Nat Commun","kind":"paper","lang":"en","published_at":"2026-11-03T00:00:00+00:00","fetched_at":"2026-11-08T03:44:12+00:00","authors":["van Biljon R","Bhatnagar S","Kumar R","Gliozzi ML","Xu G","Tripathi AK","Ashrafi A","Swearingen KE","Sinnis P","Llinás M","Painter HJ"],"primary_topic":"animal-models","topics":[{"slug":"ethics-policy","score":2.0,"hits":["regulation","regulatory"]}],"topic_score":2.0,"preference_score":0.0,"preference_labels":[],"llm_score":46.0,"final_score":54.0,"score_dims":{"novelty":66,"rigor":74,"impact":38,"relevance":22,"translation":20},"recommend_reason":"机制层面（RNA结合蛋白调控谱系特异性翻译、影响雄配子育性）对配子生物学有一定启发，但研究对象为疟原虫传播生物学，与人类生殖医学、辅助生殖及胚胎研究距离较远，不属于本领域必读内容。","keywords":["疟原虫","CCCH锌指蛋白","雄配子育性","翻译调控","RNA结合蛋白","传播阻断"],"selected":0,"story_id":130,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-top-journals","source_name":"PubMed · CNS/大子刊/四大医学刊（生殖相关）","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"nat-commun","journal_tier":"cns-sub","journal_if":14.7,"is_mock":false,"summary_zh":"该研究在疟原虫（Plasmodium falciparum）中鉴定CCCH型锌指蛋白PfMD3，探讨其在配子形成与宿主间传播中的作用。遗传敲除PfMD3后出现雄配子育性缺陷，蚊媒感染率下降。分子层面，PfMD3经特定RNA序列结合mRNA转录本，所编码的配子体蛋白在敲除株中显著减少，提示其影响翻译；此外PfMD3还与mRNA加工及核输出相关蛋白互作，参与翻译调控。研究据此将PfMD3界定为影响谱系特异性翻译与雄配子育性的RNA结合蛋白。","summary":"Sexual maturation and fertility of the malaria-causing parasite, Plasmodium spp., are critical to transmission between hosts. This developmental process involves an intricate gene expression regulatory network that drives gametocyte commitment, development, and the production of male and female gametes. Here, we characterize the role of the CCCH-type zinc-finger protein Plasmodium falciparum Male Development 3 (PfMD3) in the formation of gametes. Genetic disruption of PfMD3 reveals a male fertility defect that decreases infectivity to mosquitoes. Molecular characterization of PfMD3 reveals its binding to mRNA transcripts is associated with male gamete development through a specific RNA sequence. Gametocyte proteins encoded by these PfMD3-bound transcripts decrease significantly in PfMD3 knockout parasites, suggesting that PfMD3 impacts translation. In addition to RNA-binding, PfMD3 also interacts with proteins involved in mRNA processing and nuclear export that influence translational regulation. This study establishes PfMD3 as an RNA-binding protein that impacts lineage-specific translation and male gamete fertility.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}