{"id":31,"title":"Full-Spectrum Spectroscopic Characterization of Extracellular pH Effects on FRET-Based Genetic Voltage Indicators Expressed in Xenopus laevis Oocytes","title_zh":"细胞外pH对非洲爪蟾卵母细胞中表达的FRET型遗传编码电压指示剂影响的全光谱光谱表征","url":"https://pubmed.ncbi.nlm.nih.gov/42955629","doi":"10.1007/978-1-0716-5687-7_6","pmid":"42955629","journal":"Methods Mol Biol","kind":"paper","lang":"en","published_at":null,"fetched_at":"2026-11-08T03:43:49+00:00","authors":["Yongche Y","Okochi Y","Okamura Y","Tsutsui H"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":4.0,"hits":["oocytes"]},{"slug":"animal-models","score":3.2,"hits":["xenopus"]},{"slug":"omics-methods","score":1.0,"hits":["single cell"]}],"topic_score":4.0,"preference_score":0.0,"preference_labels":[],"llm_score":48.0,"final_score":48.0,"score_dims":{"novelty":45,"rigor":55,"impact":30,"relevance":25,"translation":30},"recommend_reason":"作为膜电位指示剂pH敏感性表征的操作方案较为规范，但研究对象为爪蟾卵母细胞表达体系，与生殖医学、辅助生殖及胚胎多组学核心问题关联很弱，仅对从事卵母细胞电生理/光学记录方法学的人员有参考价值。","keywords":["遗传编码电压指示剂","FRET","非洲爪蟾卵母细胞","pH敏感性","全光谱光谱学","电生理"],"selected":0,"story_id":null,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-embryo-omics","source_name":"PubMed · 胚胎/卵母细胞组学","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"本文为《Methods Mol Biol》的方法学章节，提供了逐步实验方案：在非洲爪蟾卵母细胞中表达FRET型遗传编码电压指示剂，利用整套测量装置（含组装与光路对准）对细胞外pH影响进行全光谱光谱分析。作者指出，pH敏感性是电压指示剂关键但常被低估的特性，在将成像拓展至细胞器膜时尤为重要；卵母细胞可在稳定的电生理控制下实现单细胞实时全光谱测量，从而获得在其他体系中难以实现的机制性表征。原文未提供具体实验数据、样本量与统计结果，发表时间亦未知，故仅为方案性描述。","summary":"Genetically encoded voltage indicators convert voltage-dependent conformational transitions of membrane proteins into optical signals. pH sensitivity is a critical yet often under-characterized property and becomes particularly important when extending imaging to organellar membranes. This chapter presents a step-by-step protocol for full-spectrum spectroscopic analysis of extracellular pH effects on FRET-based voltage indicators expressed in Xenopus laevis oocytes, beginning with assembly and alignment of the measurement rig. Oocytes enable real-time, full-spectrum spectroscopic measurements within a single cell under stable electrophysiological control, providing well-defined mechanistic characterization under conditions difficult to achieve in other systems.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}