{"id":283,"title":"A hemicyanine-based activatable near-infrared probe for cysteine and its fluorescence response in a mouse model of polycystic ovary syndrome","title_zh":"基于半花菁的可激活近红外探针用于半胱氨酸检测及多囊卵巢综合征小鼠模型荧光响应","url":"https://pubmed.ncbi.nlm.nih.gov/42986473","doi":"10.1007/s00604-026-08489-3","pmid":"42986473","journal":"Mikrochim Acta","kind":"paper","lang":"en","published_at":"2026-11-05T00:00:00+00:00","fetched_at":"2026-11-08T03:44:04+00:00","authors":["Zhao WB","Wu ZB","Zhao R","Mao LY","Liu LJ","Liu H","Zhou YH","Zeng C"],"primary_topic":"repro-endo","topics":[{"slug":"repro-endo","score":5.0,"hits":["pcos","polycystic ovary"]},{"slug":"oocyte","score":4.0,"hits":["ovary"]},{"slug":"repro-aging","score":1.0,"hits":["mitochondrial"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":58.0,"final_score":58.0,"score_dims":{"novelty":60,"rigor":55,"impact":40,"relevance":50,"translation":35},"recommend_reason":"该文提供了一种可用于PCOS相关模型Cys响应检测的近红外探针，方法学有一定新意；但生物学信号尚未独立定量归因于Cys，诊断与转化价值有限，适合作为边缘相关方法学浏览。","keywords":["半花菁探针","半胱氨酸","近红外荧光","多囊卵巢综合征","小鼠模型"],"selected":0,"story_id":46,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-endo-genetics","source_name":"PubMed · 生殖内分泌/遗传/保存","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"研究开发了丙烯酸酯笼蔽的半花菁探针HD-ZWB，用于半胱氨酸（Cys）响应的近红外荧光检测。在指定缓冲条件下，740 nm发射随1–10 µM Cys线性增强，检出限0.01 µM，约60 min达平台；对测试干扰物显示一定选择性，50 µM内细胞存活率>80%。OVCAR3细胞中激活荧光与线粒体标记重叠，并随PMA、SIN-1、LPS或N-乙酰半胱氨酸处理出现终点差异。PCOS小鼠模型中荧光15 min可检出、约30 min达峰，PCOS血清荧光约为对照5倍。作者指出结果属探索性，仍需独立Cys定量和化学验证。","summary":"Altered sulfur amino acid metabolism motivates the investigation of cysteine (Cys)-responsive fluorescence in polycystic ovary syndrome (PCOS)-related models. We developed HD-ZWB, an acrylate-caged hemicyanine probe that exhibits near-infrared fluorescence enhancement upon exposure to Cys. The response is consistent with a proposed Michael addition-cyclization pathway that releases the hemicyanine fluorophore. Under the specified buffer conditions, emission at 740 nm increased linearly over 1-10 µM Cys, with a reported detection limit of 0.01 µM, and approached a plateau within approximately 60 min. Preferential fluorescence activation by Cys was observed relative to the tested interferents. Cell viability remained above 80% at probe concentrations up to 50 µM. In OVCAR3 cells, the activated fluorescence overlapped with a mitochondrial marker and showed treatment-dependent endpoint differences following exposure to PMA, SIN-1, LPS, or N-acetylcysteine. Curcumin also reduced the endpoint signal in a high-content screen. In the PCOS mouse model, fluorescence was detectable at 15 min and peaked near 30 min; probe-treated PCOS serum showed approximately five-fold higher fluorescence than control serum. These results support exploratory fluorescence measurements with HD-ZWB in PCOS-related models. Independent Cys quantification and further chemical validation are required to assign the biological signals quantitatively to Cys, and the present data do not establish reversible sensing or diagnostic performance.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}