{"id":278,"title":"MicroRNA-205-5p: a candidate biomarker and mechanistic mediator in polycystic ovary syndrome","title_zh":"miR-205-5p：多囊卵巢综合征的候选生物标志物与机制介导者","url":"https://pubmed.ncbi.nlm.nih.gov/42989245","doi":"10.1186/s12920-026-02436-0","pmid":"42989245","journal":"BMC Med Genomics","kind":"paper","lang":"en","published_at":"2026-08-07T00:00:00+00:00","fetched_at":"2026-11-08T03:44:04+00:00","authors":["Gu X","Zhang Y","Gao R","Hao S","Wu T"],"primary_topic":"repro-endo","topics":[{"slug":"repro-endo","score":5.0,"hits":["pcos","polycystic ovary"]},{"slug":"oocyte","score":4.0,"hits":["ovary"]},{"slug":"repro-tox","score":1.0,"hits":["bmi"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":66.0,"final_score":66.0,"score_dims":{"novelty":60,"rigor":65,"impact":58,"relevance":70,"translation":62},"recommend_reason":"该研究为PCOS合并胰岛素抵抗提供了诊断性能较好的血清miRNA候选标志物，并初步验证SMAD4靶点；但为单中心回顾性设计、样本量中等、缺乏独立验证队列与机制功能实验，适合作为生物标志物线索浏览。","keywords":["多囊卵巢综合征","miR-205-5p","胰岛素抵抗","生物标志物","SMAD4"],"selected":1,"story_id":457,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-endo-genetics","source_name":"PubMed · 生殖内分泌/遗传/保存","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该回顾性研究纳入80例多囊卵巢综合征（PCOS）患者与65名健康对照，采用qRT-PCR检测血清miR-205-5p水平，并通过ROC曲线、Logistic回归及生物信息学分析评估其诊断价值与机制。结果显示，PCOS患者（尤其合并胰岛素抵抗者，IR）血清miR-205-5p显著升高，区分PCOS与对照的AUC达0.955，区分IR与非IR的AUC为0.852；其与HOMA-IR正相关（r=0.693），且为IR的独立危险因素（OR=3.849）。荧光素酶实验提示SMAD4为其潜在靶点。结论认为miR-205-5p有望作为PCOS合并IR的无创早期检测标志物。","summary":"BACKGROUND: The objective of this study was to investigate the impact of microRNA-205-5p (miR-205-5p) on clinical outcomes in patients with polycystic ovary syndrome (PCOS). METHODS: Eighty PCOS patients and sixty-five healthy individuals were enrolled in this retrospective study. Serum levels of miR-205-5p were measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The diagnostic efficacy was assessed using receiver operating characteristic (ROC) curves. Logistic regression analysis was conducted to identify the risk factors for insulin resistance (IR) in PCOS patients. Additionally, bioinformatics analysis was performed for the functional enrichment of miR-205-5p targets. RESULTS: Serum levels of miR-205-5p were significantly elevated in PCOS patients, particularly in those with IR (P < 0.001). A high diagnostic accuracy of serum miR-205-5p was displayed for distinguishing PCOS patients from healthy controls (AUC = 0.955) and for distinguishing IR from non-IR among PCOS patients (AUC = 0.852). Moreover, miR-205-5p exhibited a positive correlation with the HOMA-IR value (r = 0.6930, P < 0.001). Abnormal levels of miR-205-5p were significantly associated with BMI, FINS, FBG and HOMA-IR in PCOS patients with IR (all P < 0.001). Furthermore, miR-205-5p was identified as an independent risk factor for IR in PCOS patients, demonstrating a substantial effect size (P = 0.014; OR = 3.849;95%CI = 1.313-11.280), indicating that elevated levels of miR-205-5p were associated with a 3.849-fold increased risk of IR among this population. Bioinformatic analysis and luciferase reporter assays identified SMAD4 as a potential target of miR-205-5p. CONCLUSIONS: MiR-205-5p expression was significantly elevated in PCOS patients with IR, suggesting its potential as a non-invasive biomarker for the early detection of IR in this cohort.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}