{"id":27,"title":"RNA-seq dataset of female germline stem cells treated with sodium lactate","title_zh":"乳酸钠处理雌性生殖干细胞后的RNA-seq数据集","url":"https://pubmed.ncbi.nlm.nih.gov/42960231","doi":"10.1016/j.dib.2026.113348","pmid":"42960231","journal":"Data Brief","kind":"paper","lang":"en","published_at":"2026-10-14T00:00:00+00:00","fetched_at":"2026-11-08T03:43:49+00:00","authors":["Guo Y","Xu B","Gu JJ","Ma Y","Hu X","Wu J"],"primary_topic":"ivg-stem","topics":[{"slug":"ivg-stem","score":5.0,"hits":["germ cells","germline"]},{"slug":"omics-methods","score":5.0,"hits":["rna-seq","transcriptomic"]},{"slug":"oocyte","score":2.0,"hits":["oocytes","ovaries"]},{"slug":"ethics-policy","score":1.0,"hits":["regulation"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":60.0,"final_score":60.0,"score_dims":{"novelty":58,"rigor":50,"impact":52,"relevance":66,"translation":45},"recommend_reason":"乳酸化修饰与生殖干细胞命运的联系是较新的切入点，且提供了可复用的转录组数据集，适合关注代谢修饰与体外配子发生的实验室作为数据资源浏览；但内容为描述性数据简报，样本与验证信息有限，无机制结论，临床与ART转化价值有限。","keywords":["雌性生殖干细胞","乳酸化修饰","RNA-seq","可变剪接","GSEA","数据集"],"selected":1,"story_id":409,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-embryo-omics","source_name":"PubMed · 胚胎/卵母细胞组学","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该数据简报以小鼠来源雌性生殖干细胞（FGSC）为对象，用乳酸钠处理改变其乳酸化修饰状态，并通过RNA-seq检测乳酸化相关基因与发育相关基因的转录组变化。经数据清洗、基因组比对与统计检验，共鉴定到14,376个表达基因，其中1,827个显著上调、417个显著下调；作者进一步用GSEA富集分析和mRNA可变剪接分析刻画乳酸化改变对转录组的影响。该数据集为研究乳酸化修饰调控FGSC增殖与分化提供参考，但摘要未说明样本量、重复数与独立验证，主要发现限于差异基因清单，未给出机制性结论。","summary":"Female germline stem cells(FGSC) are a type of adult stem cell found in the ovaries of animals which possess the ability to self-renew in vitro and differentiate into mature oocytes. Lactylation is a widespread post-translational modification of proteins that plays a crucial role in biological processes such as cell fate regulation and cell signaling, and has been shown to significantly influence processes such as immunity and development. However, the effects of altered lactylation on the development of female germline stem cells remain unclear. Therefore, we used sodium lactate treatment to modify the lactylation status of mouse-derived female germline stem Y cells(Supplementary Fig. 1) and employed RNA-seq technology to detect and analyze changes in lactylation-related genes and development-related genes. Through data cleaning of sequencing reads, genome alignment, and statistical testing, a total of 14,376 expressed genes were identified, of which 1,827 were significantly upregulated and 417 were significantly downregulated. We investigated the effects of altered lactic acid modification on the transcriptomic expression of female germline stem cells using GSEA enrichment analysis and mRNA alternative splicing analysis. This dataset will help us understand the effects of lactylation on processes such as proliferation and differentiation in female germline stem cells, providing a reference for research into the development of female germ cells.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}