{"id":257,"title":"BCAT1 promotes acetylation of ACLY through fatty acid metabolism pathway promoting the occurrence and development of endometriosis","title_zh":"BCAT1通过脂肪酸代谢途径促进ACLY乙酰化，推动子宫内膜异位症发生发展","url":"https://pubmed.ncbi.nlm.nih.gov/42974315","doi":"10.3389/fgene.2026.1818692","pmid":"42974315","journal":"Front Genet","kind":"paper","lang":"en","published_at":"2026-10-20T00:00:00+00:00","fetched_at":"2026-11-08T03:44:00+00:00","authors":["Xu Y","Shen Y","Yan Y","Chen L"],"primary_topic":"endometrium","topics":[{"slug":"endometrium","score":5.0,"hits":["endometrial","endometriosis"]},{"slug":"ethics-policy","score":1.0,"hits":["regulation"]},{"slug":"omics-methods","score":1.0,"hits":["transcriptomic"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":70.0,"final_score":70.0,"score_dims":{"novelty":68,"rigor":60,"impact":62,"relevance":72,"translation":58},"recommend_reason":"选题切入内异症代谢重编程与表观调控，BCAT1/ACLY轴有一定新意；但证据以体外细胞实验为主，样本量与在体验证信息有限，适合作为机制线索浏览而非临床实践依据。","keywords":["子宫内膜异位症","BCAT1","ACLY","脂肪酸代谢","组蛋白乙酰化","异位子宫内膜间质细胞"],"selected":1,"story_id":361,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-uterus-pregnancy","source_name":"PubMed · 内膜/植入/早孕","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"front-genet","journal_tier":"standard","journal_if":2.8,"is_mock":false,"summary_zh":"该研究探讨支链氨基酸转氨酶1（BCAT1）在子宫内膜异位症（EM）中的作用。作者用qRT-PCR和Western blot检测EM组织中BCAT1表达，并通过CCK-8、克隆形成、划痕和Transwell实验评估其对异位子宫内膜间质细胞（EESC）增殖、迁移和侵袭的影响；结合转录组测序、耗氧率、脂滴及胆固醇/甘油三酯检测分析脂肪酸代谢。结果显示BCAT1在EM组织中显著升高，可促进EESC恶性样行为，并正向调控脂肪酸代谢、上调ACLY表达，机制涉及组蛋白乙酰化介导的表观调控。研究提示BCAT1/ACLY轴可能是EM潜在治疗靶点，但证据主要来自体外实验。","summary":"OBJECTIVE: Currently, surgical intervention is the primary treatment for endometriosis (EM), but recurrence is common. This study aims to identify novel potential therapeutic targets for effective management of EM by investigating the role of branched chain amino acid transaminase 1 (BCAT1). METHODS: We analyzed BCAT1 expression in EM tissues using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot analysis. The functional impact of BCAT1 on ectopic endometrial stromal cells (EESCs) was evaluated using CCK-8, colony formation, wound healing, and Transwell assays. To study BCAT1's role in fatty acid metabolism, we conducted transcriptomic sequencing, oxygen consumption rate (OCR) assays, lipid droplet assays, and measured cholesterol and triglyceride levels. The interaction between BCAT1 and ATP-citrate lyase (ACLY) was further analyzed through Western blotting and BCAT1 modulation experiments. RESULTS: BCAT1 expression was significantly elevated in EM tissues. Functional assays demonstrated that BCAT1 promotes the proliferation, migration, and invasion of EESCs in vitro. BCAT1 also positively regulates fatty acid metabolism, which enhances ACLY expression and contributes to cell proliferation. Mechanistically, BCAT1 influences ACLY expression through epigenetic regulation, specifically via histone acetylation. CONCLUSION: BCAT1 plays a crucial role in regulating EESC functions by modulating fatty acid metabolism and ACLY expression. These findings provide a theoretical basis for EM treatment.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}