{"id":224,"title":"High-resolution spatial transcriptomics reveals fibroblast and neuroimmune microenvironments in endometriosis lesions","title_zh":"高分辨率空间转录组揭示子宫内膜异位病灶中的成纤维细胞与神经免疫微环境","url":"https://pubmed.ncbi.nlm.nih.gov/42988828","doi":"10.1038/s41467-026-78453-5","pmid":"42988828","journal":"Nat Commun","kind":"paper","lang":"en","published_at":"2026-10-09T00:00:00+00:00","fetched_at":"2026-11-08T03:44:00+00:00","authors":["Haney CM","Alizadeh E","Lee J","Sullivan M","Kuljancic J","Flynn WF","Robson P","White BS","Luciano DE","Courtois ET"],"primary_topic":"endometrium","topics":[{"slug":"endometrium","score":6.0,"hits":["endometriosis","endometrium","uterus"]},{"slug":"omics-methods","score":4.0,"hits":["spatial transcriptomics","transcriptomic"]},{"slug":"oocyte","score":1.0,"hits":["ovarian"]},{"slug":"repro-immunology","score":1.0,"hits":["macrophage"]}],"topic_score":6.0,"preference_score":0.0,"preference_labels":[],"llm_score":78.0,"final_score":86.0,"score_dims":{"novelty":82,"rigor":80,"impact":75,"relevance":65,"translation":55},"recommend_reason":"首次在卵巢型与腹膜型病灶中以高分辨率空间转录组系统刻画神经-免疫-成纤维细胞微环境，并用类器官共培养验证上皮-神经元互作，是理解内异症疼痛机制的重要一手证据；但与ART实验室日常决策距离较远，转化尚处早期。","keywords":["子宫内膜异位症","空间转录组","神经免疫微环境","成纤维细胞","感觉神经元","类器官共培养"],"selected":1,"story_id":417,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-uterus-pregnancy","source_name":"PubMed · 内膜/植入/早孕","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"nat-commun","journal_tier":"cns-sub","journal_if":14.7,"is_mock":false,"summary_zh":"研究对卵巢型与腹膜型子宫内膜异位病灶进行高分辨率空间转录组制图，发现两类病灶共有的空间特征：免疫细胞浸润、环绕腺上皮的成纤维细胞区室，以及神经元亚群与巨噬细胞亚群的特异性分布。作者进一步精确定义了感觉神经元亚型，并标定其与免疫细胞的空间毗邻关系；并利用外周感觉脑类器官与人子宫内膜异位上皮及成纤维细胞共培养的体外3D模型，验证了上皮-神经元互作组。该工作从空间层面刻画了与疼痛相关的细胞互作与保守特征，为病理生理理解与靶向治疗开发提供依据。","summary":"Endometriosis is a chronic, systemic, inflammatory disease characterized by the presence of endometrium-like tissue growing outside of the uterus. Affecting one in ten individuals assigned female at birth, it causes chronic pain and inflammation that significantly impairs quality of life. Yet the mechanisms that drive pain and disease progression remain poorly defined. This study establishes the precise spatial transcriptomic cartography of human ovarian and peritoneal lesions, two of the most commonly found lesions. We identify shared spatial features across lesion types, including immune cell infiltration, fibroblast compartments surrounding epithelial glands, and distinct distributions of neuronal and macrophage subsets. We precisely defined sensory neuronal subtypes and mapped their spatial location relative to immune cells. We further validate the epithelial-neuronal interactome, using an in vitro 3D model of peripheral sensory brain organoids co-cultured with human endometriosis epithelial and fibroblast cells. By mapping spatial cellular interactions and conserved features across lesion types linked to pain, our study advances understanding of endometriosis pathophysiology and informs targeted therapeutic development.","interpretation_basis":null,"fulltext_status":"not_found","interpretation":null,"fulltext":{"available":false,"status":"not_found","reason":"Europe PMC 无此记录：DOI 10.1038/s41467-026-78453-5","pmcid":null,"license":null,"chars":0,"source":"Europe PMC","fetched_at":"2026-11-08T04:19:47+00:00"}}