{"id":203,"title":"Evaluation of miR-155-5p expression and ovarian histopathological changes in a cyclophosphamide-induced mouse model of premature ovarian insufficiency","title_zh":"环磷酰胺诱导的早发性卵巢功能不全小鼠模型中 miR-155-5p 表达与卵巢组织病理学改变的评估","url":"https://pubmed.ncbi.nlm.nih.gov/42967256","doi":"10.1007/s10735-026-11025-5","pmid":"42967256","journal":"J Mol Histol","kind":"paper","lang":"en","published_at":"2026-11-01T00:00:00+00:00","fetched_at":"2026-11-08T03:43:57+00:00","authors":["Saadatian Z","Navid S","Talebi A","Khajavian N","Chalaki HB"],"primary_topic":"repro-endo","topics":[{"slug":"repro-endo","score":10.0,"hits":["amenorrhea","amh","anti-müllerian","estradiol","fsh","poi","premature ovarian insufficiency"]},{"slug":"oocyte","score":8.0,"hits":["follicle","follicles","folliculogenesis","oocyte","ovarian"]},{"slug":"fertility-preservation","score":1.0,"hits":["ovarian tissue"]}],"topic_score":10.0,"preference_score":5.0,"preference_labels":["可转化到 ART 实验室的发现"],"llm_score":62.0,"final_score":67.0,"score_dims":{"novelty":55,"rigor":60,"impact":55,"relevance":80,"translation":50},"recommend_reason":"属于生殖内分泌与卵泡生物学的常规动物模型描述性研究，样本量小（每组 8 只）、仅相关未做功能验证，机制与转化价值有限；对关注 POI 生物标志物与化疗性卵巢损伤的研究者可作背景浏览。","keywords":["早发性卵巢功能不全","miR-155-5p","环磷酰胺","AMH","GDF9","卵巢组织病理"],"selected":1,"story_id":237,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"研究在环磷酰胺诱导的早发性卵巢功能不全（POI）小鼠模型中评估 miR-155-5p 及卵泡发育相关基因 AMH、GDF9 的表达。16 只雌性 NMRI 小鼠随机分为模型组与对照组（各 8 只），模型组腹腔注射环磷酰胺 120 mg/kg。QRT-PCR 显示模型组 miR-155-5p 显著上调（P<0.001）、AMH 显著下调（P<0.001）、GDF9 上调（P<0.05）；H&E、甲苯胺蓝与 PAS 染色见健康卵泡减少、闭锁增多、间质破坏及卵母细胞退变；血清雌二醇下降、FSH 升高。作者认为 miR-155-5p 可能作为卵巢损伤的候选生物标志物，但尚未证实因果关系。","summary":"BACKGROUND: Premature ovarian insufficiency (POI) is a reproductive disorder characterized by amenorrhea, reduced estrogen levels, and elevated gonadotropins. Increasing evidence highlights the involvement of microRNAs, particularly miR-155-5p, in inflammation and apoptosis. This study aimed to evaluate the expression level of miR-155-5p and key folliculogenesis genes. Anti-Müllerian hormone(AMH) and growth differentiation factor 9 (GDF9) in the ovarian tissue of a cyclophosphamide-induced POI mouse model compared with healthy controls, alongside histopathological assessment and follicle quantification. METHODS: Sixteen female NMRI mice were randomly assigned to two groups (n = 8 each). The experimental group received intraperitoneal cyclophosphamide (120 mg/kg) to induce POI, while the control group received normal saline. Ovarian tissues were harvested for molecular and histological analyses. Total RNA was extracted and subjected to cDNA synthesis, and miR-155-5p, AMH, and GDF9 expression were quantified by QRT-PCR. For morphological evaluation, serial ovarian sections were stained with hematoxylin-eosin (H&E), toluidine blue, and Periodic Acid-Schiff (PAS) special stains. RESULTS: miR-155-5p expression was significantly upregulated in the POI group compared with controls (P < 0.001). AMH expression was markedly downregulated (P < 0.001) and GDF9 expression was significantly upregulated (P < 0.05). Histopathology revealed reduced healthy follicles, increased atresia, stromal disruption, and oocyte degeneration in the POI group. Serum estradiol decreased, while FSH levels increased. CONCLUSION: Upregulation of miR-155-5p was associated with ovarian histopathological alterations and changes in folliculogenesis-related gene expression in the cyclophosphamide-induced POI model. These findings identify miR-155-5p as a potential candidate biomarker of ovarian injury in this experimental setting. However, the present findings do not establish a causal or mechanistic role for miR-155-5p. Further functional studies, including gain- and loss-of-function experiments and target validation, as well as clinical studies, are required to determine its biological and clinical significance.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}