{"id":2,"title":"Integrative mRNA and lncRNA transcriptome analysis of enhanced developmental competence of bovine GV stage oocytes vitrified in liquid helium","title_zh":"液氦玻璃化牛GV期卵母细胞的mRNA与lncRNA整合转录组分析揭示发育能力提升相关调控网络","url":"https://pubmed.ncbi.nlm.nih.gov/42994184","doi":"10.1016/j.theriogenology.2026.118314","pmid":"42994184","journal":"Theriogenology","kind":"paper","lang":"en","published_at":"2026-11-04T00:00:00+00:00","fetched_at":"2026-11-08T03:43:49+00:00","authors":["Li X","Yan Q","Yuan B","Zhang J","Zhao S","Wang Q","Bai H","Ren M","Cui Y","Hu H","Gao Q","Zhang Y","Ma W","Wen F","Xu Z"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":8.0,"hits":["germinal vesicle","in vitro maturation","meiotic","oocyte","oocytes"]},{"slug":"embryology-lab","score":6.0,"hits":["cryopreservation","vitrification","vitrified"]},{"slug":"omics-methods","score":4.0,"hits":["transcriptome","transcriptomic"]},{"slug":"early-embryo","score":1.0,"hits":["blastocyst"]},{"slug":"art-clinical","score":1.0,"hits":["in vitro fertilization"]},{"slug":"fertility-preservation","score":1.0,"hits":["oocyte cryopreservation"]},{"slug":"ethics-policy","score":1.0,"hits":["regulatory"]}],"topic_score":8.0,"preference_score":17.0,"preference_labels":["早期胚胎转录组/单细胞转录组","可转化到 ART 实验室的发现"],"llm_score":72.0,"final_score":89.0,"score_dims":{"novelty":68,"rigor":70,"impact":65,"relevance":82,"translation":70},"recommend_reason":"将液氦玻璃化与牛GV期卵母细胞转录组网络联系起来，为卵母细胞冷冻保存优化提供候选分子线索；但样本量和验证深度有限，且为动物模型，临床转化需谨慎外推。","keywords":["液氦玻璃化","卵母细胞","lncRNA","转录组","冷冻保存","牛"],"selected":1,"story_id":109,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-embryo-omics","source_name":"PubMed · 胚胎/卵母细胞组学","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"theriogenology","journal_tier":"standard","journal_if":2.8,"is_mock":false,"summary_zh":"该研究比较液氦（LHe）与液氮（LN）玻璃化牛GV期卵母细胞的复苏后发育能力，并进行mRNA和lncRNA转录组测序、功能富集、共表达网络分析及RT-qPCR验证。结果显示LHe组卵裂率（43.0% vs 33.6%）和囊胚率（12.1% vs 8.5%）显著提高；鉴定出361个差异mRNA和137个差异lncRNA，涉及免疫应答、代谢、有丝分裂DNA完整性检查点及同源染色体分离等通路，并预测HORMAD1、CDYL、TRIOBP为潜在核心枢纽基因。结论提示LHe玻璃化可能通过特定mRNA-lncRNA共表达网络改善卵母细胞减数分裂完整性和应激适应。","summary":"Liquid helium (LHe) vitrification improves the post-thaw developmental competence of bovine germinal vesicle (GV) stage oocytes; however, the underlying key transcriptomic regulatory networks still unexplored. This study aimed to evaluate the mRNA and lncRNA transcriptomic and developmental competence of bovine GV stage oocytes vitrified with LHe versus liquid nitrogen (LN). Bovine GV-stage oocytes were vitrified using LHe or LN as the cryogen. Post-thaw developmental competence was assessed following in vitro maturation, in vitro fertilization, and in vitro culture. High-throughput sequencing was performed to profile mRNA and lncRNA expressions between the oocytes vitrified in LHe and LN, followed by functional enrichment and co-expression network analyses. The transcriptomic alterations were independently validated by RT-qPCR. The present study found that LHe vitrification significantly improved cleavage rate (43.0% vs. 33.6%) and blastocyst rate (12.1% vs. 8.5%) compared to LN (p < 0.05). Transcriptomic analysis identified 361 differentially expressed mRNAs and 137 differentially expressed lncRNAs between the two groups. GO and KEGG analyses revealed that these mRNAs were enriched in immune response and metabolic pathways, while the lncRNA target genes were associated with the mitotic DNA integrity checkpoint and homologous chromosome segregation. In silico co-expression network analysis predicted HORMAD1, CDYL, and TRIOBP as potential central hub genes. In conclusion, LHe vitrification enhances the developmental competence of bovine GV-stage oocytes, which may associate with distinct mRNA-lncRNA co-expression networks and specific molecular signatures related to meiotic integrity and stress adaptation. These findings provide valuable transcriptomic resources and potential biomarkers for optimizing oocyte cryopreservation.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}