{"id":175,"title":"Effects of intraperitoneal administration of kisspeptin-10 on ischemia-reperfusion injury in a rat testicular torsion and detorsion model","title_zh":"腹腔注射kisspeptin-10对大鼠睾丸扭转-复位模型中缺血再灌注损伤的影响","url":"https://pubmed.ncbi.nlm.nih.gov/42979496","doi":"10.30466/vrf.2025.2068679.4873","pmid":"42979496","journal":"Vet Res Forum","kind":"paper","lang":"en","published_at":"2026-08-15T00:00:00+00:00","fetched_at":"2026-11-08T03:43:57+00:00","authors":["Karvani Dilmaghani N","Behfar M","Soleimanzadeh A","Davoodi F"],"primary_topic":"male","topics":[{"slug":"male","score":7.0,"hits":["sperm","sperm dna","testicular","testis"]},{"slug":"repro-endo","score":4.0,"hits":["kisspeptin"]}],"topic_score":7.0,"preference_score":0.0,"preference_labels":[],"llm_score":56.0,"final_score":56.0,"score_dims":{"novelty":58,"rigor":45,"impact":48,"relevance":62,"translation":40},"recommend_reason":"选题聚焦男性生殖氧化应激损伤与kisspeptin的潜在保护作用，思路有一定新意；但仅20只大鼠、缺乏 sham 组与分子机制验证，发表在兽医类期刊，与ART临床距离较远，可作为机制探索的背景阅读。","keywords":["kisspeptin-10","睾丸扭转","缺血再灌注损伤","精子DNA损伤","氧化应激","大鼠模型"],"selected":0,"story_id":444,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该研究在大鼠睾丸扭转/复位（720°扭转2小时）模型中，评估腹腔给予kisspeptin-10（Kiss-10，0.50与1.00 µg/kg）对缺血再灌注损伤的保护作用。20只雄性大鼠分为对照组与三个处理组，检测精子数量、活力、存活率、DNA损伤及低渗肿胀试验。结果显示，720°扭转/复位显著增加精子DNA损伤，并损害总活力、存活率与质膜功能；给予Kiss-10可降低DNA损伤，并改善总活力、存活率与质膜功能。作者认为，Kiss-10在精索扭转后给药对急性睾丸扭转/复位损伤具有明显保护作用。该研究为动物实验，样本量小，结果需进一步验证。","summary":"Testicular ischemia-reperfusion injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of uncaptured reactive oxygen species. Kisspeptin-10 (Kiss-10), a biologically active fragment of kisspeptin peptide family, has demonstrated significant anti-oxidant and anti-apoptotic properties. Recent studies indicate that Kiss-10 can mitigate oxidative stress by reducing reactive oxygen species levels and enhancing the activity of endogenous anti-oxidant enzymes, such as superoxide dismutase and catalase. This study examined the effects of Kiss-10on ischemia-reperfusion injury in testicular torsion/detorsion (T/D) of male rats. Twenty male rats were divided into four groups, including the control group and three treatment groups (720° T/D, 720° T/D + 0.50 µg kg-1 Kiss-10, and 720° T/D + 1.00 µg kg-1 Kiss-10). After inducing 720° clockwise testicular torsion for 2 hr, sperm parameters, such as number, motility, viability, DNA damage, and hypo-osmotic test were evaluated. The results showed that 720° T/D can increase sperm DNA damage. Also, it also had negative effects on overall motility and other properties, such as viability and plasma membrane functionality. The results also showed that administration of Kiss-10to T/D rats can reduce DNA damage. It could also increase overall motility, viability, and plasma membrane functionality compared to the T/D group. Based on our results, Kiss-10 provides significant protection against acute T/D injury to the testis when administered after spermatic cord torsion in rat.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}