{"id":163,"title":"Evaluation of dietary supplementation with Bindii (Tribulus terrestris L.), Carob (Ceratonia siliqua L.), and Ginger (Zingiber officinale Roscoe) on rooster's cooled semen quality and fertility potential during the post-peak phase","title_zh":"日粮添加蒺藜、角豆与生姜对采精后期种公鸡冷藏精液质量与受精潜力的影响","url":"https://pubmed.ncbi.nlm.nih.gov/42981632","doi":"10.1016/j.theriogenology.2026.118311","pmid":"42981632","journal":"Theriogenology","kind":"paper","lang":"en","published_at":"2026-11-03T00:00:00+00:00","fetched_at":"2026-11-08T03:43:57+00:00","authors":["Moftakharzadeh SA","Masoudi R","Barfourooshi HJ","Hosseini SA","Ghamsari AHA","Asadzadeh N","Behrouzi-Lak MA","Hajati H","Khorrami S","Sharafi M"],"primary_topic":"male","topics":[{"slug":"male","score":5.0,"hits":["semen","sperm"]},{"slug":"contraception-health","score":1.0,"hits":["fertility rates"]},{"slug":"repro-aging","score":1.0,"hits":["mitochondrial"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":60.0,"final_score":60.0,"score_dims":{"novelty":58,"rigor":60,"impact":35,"relevance":40,"translation":38},"recommend_reason":"设计规范、终点指标较全面（活力、膜完整性、脂质过氧化、线粒体活性、受精率），但研究对象为家禽、样本量小（20只），对人和ART实验室的直接转化价值有限，主要作为抗氧化饲料添加剂与冷藏精液保护的比较生物学参考。","keywords":["种公鸡","冷藏精液","植物添加剂","脂质过氧化","受精率","蒺藜/角豆/生姜"],"selected":1,"story_id":154,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"theriogenology","journal_tier":"standard","journal_if":2.8,"is_mock":false,"summary_zh":"研究将20只处于采精后期的种公鸡分为4组，分别饲喂基础日粮（对照）或添加蒺藜（1.5 g/kg）、角豆（15 g/kg）、生姜（10 g/kg）粉的日粮，持续60天。精液经腹部按摩采集、稀释后于5℃冷藏48 h，检测0、24、48 h的精子活力、活率、膜完整性、脂质过氧化与线粒体活性，并用0 h及冷藏24 h精液行人工授精评估受精率与孵化率。结果显示三种草药均提高0 h与24 h的总活力、前向运动、活率与膜完整性，降低24/48 h脂质过氧化并提升总抗氧化能力；冷藏24 h精液的受精率亦显著高于对照。结论认为三种草药可作为改善公鸡冷藏精液质量与受精力的饲料添加剂。","summary":"The current study was designed to investigate the effect of dietary supplementation with Bindii (Tribulus terrestris L.), Carob (Ceratonia siliqua L.), and Ginger (Zingiber officinale Roscoe) on reproduction parameters of cooled sperm of breeding roosters during the post-peak phase. For this purpose, 20 roosters were assigned to four equal groups and fed the following diets for 60 days: the control group (basal diet with no herbal plant supplementation), the other groups received Bindii (1.5 g/kg diet), Carob (15 g/kg diet), and Ginger (10 g/kg diet) powders as feed additives. On the 60th day, semen samples were collected via abdominal massage twice a week and diluted. Diluted semen samples were cooled to 5°C and stored for 48 h. Sperm motility, viability, membrane integrity, lipid peroxidation, and mitochondrial activity were evaluated in 0-h diluted pooled semen before cooled storage and after 24 and 48 h of storage at 5°C. Fertility and hatchability were evaluated using 0-h diluted pooled semen and semen stored at 5°C for 24 h. Results showed that feeding all herbal plants resulted in higher sperm total motility, progressive motility, viability, and membrane integrity than the control group in 0-h diluted pooled semen and after 24 h of storage (P < 0.05). However, sperm mitochondrial activity was improved by all herbal supplementation only at 24 h of storage. While groups fed herbs showed lower lipid peroxidation than those fed only the basal diet at 24 and 48 h of storage, the total antioxidant capacity was improved in 0-h diluted pooled semen and after 24 and 48 h of storage (P < 0.05). Fertility rates during artificial insemination using sperm cells cooled for 24 h were significantly higher for roosters fed all herbs than those receiving a diet without herbal plants (P < 0.05). In conclusion, the application of Bindii, Carob, and Ginger as herbal additives in a rooster diet could be an effective method to improve rooster sperm quality and fertility potential of cooled sperm.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}