{"id":159,"title":"CFAP47 is involved in sperm head-tail coupling via cooperating with Septin12 and SUN5: gene knockout and protein analysis","title_zh":"CFAP47与Septin12及SUN5协同参与精子头尾连接：基因敲除与蛋白分析","url":"https://pubmed.ncbi.nlm.nih.gov/42983223","doi":"10.1071/rd26101","pmid":"42983223","journal":"Reprod Fertil Dev","kind":"paper","lang":"en","published_at":null,"fetched_at":"2026-11-08T03:43:57+00:00","authors":["Liu T","Su Y","Liu G","Huang L","Liu X","Xu X","Xing X"],"primary_topic":"male","topics":[{"slug":"male","score":4.0,"hits":["sperm"]},{"slug":"animal-models","score":0.8,"hits":["knockout mice"]}],"topic_score":4.0,"preference_score":0.0,"preference_labels":[],"llm_score":79.0,"final_score":79.0,"score_dims":{"novelty":80,"rigor":75,"impact":75,"relevance":90,"translation":65},"recommend_reason":"该研究通过体内敲除与蛋白互作证据，将CFAP47纳入精子头尾连接机制，对MMAF及男性不育遗传诊断有参考价值；但摘要未提供样本量与统计细节，临床转化尚早。","keywords":["CFAP47","精子头尾连接","Septin12","SUN5","男性不育","MMAF"],"selected":1,"story_id":4,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该研究利用Cfap47敲除小鼠模型，结合分子对接、免疫共沉淀和邻近连接实验，探究CFAP47在精子头尾连接中的作用。结果显示，敲除小鼠精子活力受损，约70%精子出现头尾连接异常或断头；CFAP47与Septin12、SUN5协同，其缺失降低相关蛋白表达并影响这些蛋白在精子头尾连接处的定位。结论提示CFAP47是精子头尾耦合的关键分子，为男性不育机制提供线索。","summary":"CONTEXT: Sperm head-tail linkage is essential for maintaining sperm integrity and male fertility. Although CFAP47 is associated with multiple morphological abnormalities of the sperm flagella, its role in sperm head-tail coupling remains unclear. AIMS: This study aimed to investigate the role of CFAP47 in sperm head-tail connection. METHODS: A Cfap47 knockout mouse model was established to assess sperm motility and morphology. Molecular docking, co-immunoprecipitation and proximity ligation assays were used to investigate the relationships of CFAP47, Septin12 and SUN5. KEY RESULTS: Cfap47 knockout mice exhibited impaired sperm motility and severe sperm morphological defects, with approximately 70% of sperm displaying abnormal head-tail connection or decapitation. Co-immunoprecipitation and proximity ligation assays demonstrated that CFAP47 cooperates with Septin12 and SUN5. CFAP47 deficiency reduced the protein expression levels and affected their localization at the sperm head-tail junction. CONCLUSIONS: CFAP47 is essential for sperm head-tail coupling. IMPLICATIONS: These findings provide new insight into the molecular basis of sperm head-tail connection.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}