{"id":152,"title":"Integration of mRNA-seq and miRNA-seq revealed the microRNAs-transcription factors-mRNAs regulatory network in the follicular layer during oocyte maturation in Epinephelus coioides","title_zh":"整合mRNA-seq与miRNA-seq揭示卵母细胞成熟过程中滤泡层miRNA-转录因子-mRNA调控网络（以斜带石斑鱼为模型）","url":"https://pubmed.ncbi.nlm.nih.gov/42987487","doi":"10.1016/j.anireprosci.2026.108372","pmid":"42987487","journal":"Anim Reprod Sci","kind":"paper","lang":"en","published_at":"2026-11-03T00:00:00+00:00","fetched_at":"2026-11-08T03:43:57+00:00","authors":["Zhang M","Diao D","Wang Y","Tang Y","Miao Y","Yang Y","Liu S","Wu J","Zhang Y","Li S"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":9.0,"hits":["follicular","oocyte","oocyte maturation"]},{"slug":"ethics-policy","score":5.0,"hits":["regulation","regulatory"]},{"slug":"repro-aging","score":1.0,"hits":["mitochondrial"]}],"topic_score":9.0,"preference_score":0.0,"preference_labels":[],"llm_score":66.0,"final_score":66.0,"score_dims":{"novelty":68,"rigor":70,"impact":45,"relevance":42,"translation":40},"recommend_reason":"多组学整合加双荧光素酶验证的调控网络构建较完整，对卵泡层在卵母细胞成熟中的作用提供了新机制线索；但研究对象为海水养殖鱼类，与人类ART临床决策距离较远，主要价值在于比较生殖生物学与水产育种。","keywords":["卵母细胞成熟","滤泡层","miRNA-转录因子-mRNA网络","AQP1a","斜带石斑鱼","多组学整合"],"selected":1,"story_id":155,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"研究以斜带石斑鱼（Epinephelus coioides）为模型，联合mRNA-seq与miRNA-seq系统刻画卵母细胞成熟期间滤泡层的转录动态，发现明显的双相调控模式：成熟早期基础翻译机器受抑、线粒体氧化磷酸化增强，晚期则以细胞周期调控与结构重塑通路激活为主。作者构建miRNA-转录因子-mRNA多层调控网络，识别HSD17B1、VIPR1、IGFBP2等枢纽基因，并用双荧光素酶报告实验证实novel-miR-88与miR-338-3p直接靶向抑制AQP1a，提出维持滤泡结构稳定的miRNA“刹车”机制。结论为海水养殖鱼类人工繁殖与卵质改良提供候选分子靶点。","summary":"Oocyte hydration is a critical physiological event determining egg quality and reproductive success in marine teleosts that produce pelagic eggs; however, the functional contribution of the surrounding follicular layer remains largely unexplored. In this study, we used the orange-spotted grouper (Epinephelus coioides) as a model to systematically characterize the transcriptional dynamics of the follicular layer during oocyte maturation. Integrated mRNA-seq and miRNA-seq analyses demonstrated a pronounced biphasic transcriptional regulatory pattern in the follicular layer during oocyte maturation. Early maturation was characterized by suppression of basal translational machinery and enhancement of mitochondrial oxidative phosphorylation, whereas late maturation was marked by the activation of cell cycle regulation and structural remodeling pathways. By constructing a multi-layered miRNA-transcription factor (TF)-mRNA regulatory network, we identified several key hub genes, including HSD17B1, VIPR1, and IGFBP2, which are involved in steroidogenic transitions and local signaling regulation. Notably, dual-luciferase reporter assays confirmed that novel-miR-88 and miR-338-3p directly target and repress AQP1a, revealing a crucial miRNA-mediated \"braking\" mechanism that maintains follicular structural stability. Collectively, this study provides valuable molecular targets for improving artificial reproduction and egg quality in marine aquaculture species.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}