{"id":151,"title":"A one-step C-type natriuretic peptide, 17β-estradiol and amphiregulin workflow for bovine oocyte maturation","title_zh":"一步法C型利钠肽、17β-雌二醇与双调蛋白流程用于牛卵母细胞成熟","url":"https://pubmed.ncbi.nlm.nih.gov/42987801","doi":"10.1071/rd26222","pmid":"42987801","journal":"Reprod Fertil Dev","kind":"paper","lang":"en","published_at":null,"fetched_at":"2026-11-08T03:43:57+00:00","authors":["Pytel AT","Tobolski D","Gajewski Z","Papis K"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":7.0,"hits":["cumulus","in vitro maturation","ivm","oocyte","oocyte maturation"]},{"slug":"repro-endo","score":4.0,"hits":["estradiol"]},{"slug":"early-embryo","score":2.0,"hits":["blastocyst","blastocysts"]}],"topic_score":7.0,"preference_score":0.0,"preference_labels":[],"llm_score":70.0,"final_score":70.0,"score_dims":{"novelty":72,"rigor":70,"impact":60,"relevance":72,"translation":65},"recommend_reason":"以较大样本量（3182枚COC）证明CNP-E2可直接并入常规24 h IVM，省去预成熟转移步骤并提升囊胚产出，对IVP实验室流程简化有直接参考价值；但为牛模型、单一实验室，尚无胚胎质量与移植结局验证。","keywords":["C型利钠肽","17β-雌二醇","双调蛋白","卵母细胞体外成熟","牛IVP","囊胚率"],"selected":1,"story_id":5,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-gametes","source_name":"PubMed · 配子与性腺","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"该研究在牛卵母细胞体外成熟（IVM）体系中开发“一步法”方案：在常规24 h IVM中直接加入C型利钠肽（CNP）与17β-雌二醇（E2），以避免额外的预成熟培养与转移步骤。利用3182枚屠宰场来源卵丘-卵母细胞复合体开展两项实验：实验1比较常规IVM、仅回收期CNP、6 h CNP-E2预成熟后24 h IVM（双相）及24/30 h CNP-E2一步法；实验2在一步法24 h基础上于6 h后加入双调蛋白（AREG）。结果显示双相与一步法24 h均提高第8天总囊胚率（26.5%、25.7% vs 19.7%），一步法24 h亦提高第7天囊胚率（31.6% vs 23.8%），加AREG进一步提高第8天孵化囊胚率（16.0% vs 9.9%）。结论认为一步法可省去预成熟转移步骤并改善囊胚终点指标，但尚需胚胎质量与移植结局的外部验证。","summary":"CONTEXT: C-type natriuretic peptide (CNP)-based pre-maturation can improve bovine oocyte developmental competence but adds a separate culture and transfer step to in vitro embryo production (IVP). AIMS: To develop a one-step CNP and 17β-estradiol (E2) modification of conventional in vitro maturation (IVM), demonstrate its application, and assess timed amphiregulin (AREG) supplementation. METHODS: Two experiments used 3182 abattoir-derived cumulus-oocyte complexes. Experiment 1 compared conventional 24-h IVM (control), CNP during recovery only (recovery-only), 6-h CNP-E2 pre-IVM followed by 24-h IVM (biphasic), and 24- or 30-h CNP-E2 IVM (one-step 24-h and one-step 30-h). Experiment 2 compared control, one-step 24-h, and one-step 24-h + AREG, with AREG added after 6 h. Cleavage and blastocyst development were assessed on Days 3 and 7-8, respectively. KEY RESULTS: In Experiment 1, biphasic and one-step 24-h increased Day 8 total blastocyst yield relative to control (26.5% vs 19.7%, P = 0.022; and 25.7% vs 19.7%, P = 0.042, respectively). In Experiment 2, one-step 24-h increased Day 7 total blastocyst yield (31.6% vs 23.8%; P = 0.017). One-step 24-h + AREG increased Day 8 hatching blastocysts relative to control (16.0% vs 9.9%; P = 0.013) but not relative to one-step 24-h. CONCLUSIONS: Direct CNP-E2 supplementation during 24-h IVM eliminated the separate pre-IVM transfer while improving blastocyst endpoints relative to conventional IVM. IMPLICATIONS: The modification preserves a conventional 24-h IVM schedule; however, external validation using embryo-quality and post-transfer outcomes is warranted.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}