{"id":13,"title":"Low-input lipidomics reveals stage-specific lipid remodeling and differential effects of phosphatidylcholine and eicosapentaenoic acid during ovine oocyte maturation","title_zh":"低起始量脂质组学揭示绵羊卵母细胞成熟过程中的阶段特异性脂质重塑及磷脂酰胆碱与二十碳五烯酸的不同作用","url":"https://pubmed.ncbi.nlm.nih.gov/42976728","doi":"10.1016/j.theriogenology.2026.118297","pmid":"42976728","journal":"Theriogenology","kind":"paper","lang":"en","published_at":"2026-10-27T00:00:00+00:00","fetched_at":"2026-11-08T03:43:49+00:00","authors":["Zhang Y","Li W","Peng J","Lu J","Chen K","Gao L","Su J"],"primary_topic":"oocyte","topics":[{"slug":"oocyte","score":10.0,"hits":["germinal vesicle","ivm","meiotic","metaphase ii","oocyte maturation","oocytes","spindle"]},{"slug":"omics-methods","score":10.0,"hits":["lipidomic","lipidomics","low-input","mass spectrometry"]},{"slug":"animal-models","score":3.2,"hits":["ovine"]}],"topic_score":10.0,"preference_score":20.0,"preference_labels":["早期胚胎/卵母细胞蛋白质组"],"llm_score":72.0,"final_score":92.0,"score_dims":{"novelty":74,"rigor":66,"impact":63,"relevance":82,"translation":68},"recommend_reason":"首次以低起始量脂质组学系统描绘绵羊卵母细胞成熟各阶段脂质重塑，并给出PC与EPA补充对IVM成熟率的差异化效应，对卵母细胞脂质代谢与IVM培养优化有直接参考价值；但属动物模型探索性研究，样本与机制验证有限。","keywords":["卵母细胞成熟","低起始量脂质组学","磷脂酰胆碱","二十碳五烯酸","脂滴","绵羊IVM"],"selected":1,"story_id":315,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-embryo-omics","source_name":"PubMed · 胚胎/卵母细胞组学","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":"theriogenology","journal_tier":"standard","journal_if":2.8,"is_mock":false,"summary_zh":"研究采用脂质染色与UHPLC-MS/MS低起始量脂质组学，刻画绵羊卵母细胞体外成熟过程中的脂质动态。成熟MII卵母细胞的BODIPY 493/503与油红O信号高于GV期，脂滴分布更均匀；620个注释脂质中，MII期部分PC、PE、PI种类丰度较高，部分PG种类及FA 20:5较低。补充实验中，PC可提高形态学低质量C级卵母细胞的核成熟率并伴随脂质荧光升高，对A/B级无显著作用；外源EPA降低MII成熟率，300 μM EPA与异常纺锤体形态比例升高相关。结论为探索性阶段脂质图谱，PC与EPA在所用IVM条件下效应不同，支持进一步研究脂质补充策略。","summary":"Lipid metabolism is essential for oocyte maturation, but its stage-associated remodeling in ovine oocytes remains poorly defined. Here, we characterized lipid dynamics during ovine oocyte maturation using lipid staining and ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based low-input lipidomics. Mature metaphase II (MII) oocytes showed higher BODIPY 493/503 and Oil Red O staining signals than germinal vesicle (GV)-stage oocytes, whereas lipid droplet distribution was more uniform in MII oocytes than in oocytes that failed to complete maturation (OMF). Exploratory lipidomic analysis of 620 annotated entries suggested higher abundances of selected phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) entries, and lower abundances of selected phosphatidylglycerol (PG) entries and fatty acid (FA) 20:5 in MII-stage oocytes compared with GV-stage oocytes. In supplementation experiments, PC increased nuclear maturation in morphologically lower-quality Grade C oocytes and was accompanied by higher lipid-associated fluorescence in the resulting MII oocytes. No statistically significant increase in nuclear maturation was detected in Grade A/B oocytes. In contrast, exogenous eicosapentaenoic acid (EPA) supplementation reduced MII maturation rates, while no statistically significant difference in meiotic resumption was detected. Exposure to 300 μM EPA was associated with a higher proportion of oocytes with abnormal spindle morphology. These findings describe exploratory stage-associated lipid patterns during ovine oocyte maturation and show distinct effects of exogenous PC and EPA supplementation under the tested IVM conditions, supporting further investigation of lipid supplementation strategies for ovine IVM.","interpretation_basis":null,"fulltext_status":"not_found","interpretation":null,"fulltext":{"available":false,"status":"not_found","reason":"Europe PMC 无此记录：DOI 10.1016/j.theriogenology.2026.118297","pmcid":null,"license":null,"chars":0,"source":"Europe PMC","fetched_at":"2026-11-08T03:59:53+00:00"}}