{"id":120,"title":"Effects of a simplified hormonal treatment comprising a single FSH administration and induction of follicular wave emergence by CIDR and estradiol benzoate prior to ovum pick-up on IVP and embryo transfer outcomes in Japanese Black cows","title_zh":"简化激素方案：单次FSH注射联合CIDR与苯甲酸雌二醇诱导卵泡波对日本黑牛OPU-IVP及胚胎移植结局的影响","url":"https://pubmed.ncbi.nlm.nih.gov/42968260","doi":"10.1262/jrd.2026-105","pmid":"42968260","journal":"J Reprod Dev","kind":"paper","lang":"en","published_at":"2026-11-02T00:00:00+00:00","fetched_at":"2026-11-08T03:43:53+00:00","authors":["Okami K","Ho TC","Nabenishi H","Momozawa K","Nagano M"],"primary_topic":"repro-endo","topics":[{"slug":"repro-endo","score":9.0,"hits":["estradiol","follicle-stimulating hormone","fsh","progesterone"]},{"slug":"oocyte","score":7.0,"hits":["follicle","follicular","oocyte quality","oocytes"]},{"slug":"art-clinical","score":4.0,"hits":["embryo transfer"]},{"slug":"early-embryo","score":1.0,"hits":["blastocysts"]}],"topic_score":9.0,"preference_score":5.0,"preference_labels":["可转化到 ART 实验室的发现"],"llm_score":71.0,"final_score":76.0,"score_dims":{"novelty":70,"rigor":55,"impact":62,"relevance":72,"translation":68},"recommend_reason":"该方案操作简化且同时改善卵母细胞质量、囊胚产量与移植妊娠率，对牛OPU-IVP实验室有直接参考价值；但样本量小（每组6头）且为动物研究，外推到人类ART需谨慎。","keywords":["日本黑牛","OPU-IVP","卵泡波同步","FSH","CIDR","胚胎移植"],"selected":1,"story_id":203,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":1,"has_interpretation":false,"journal_key":"j-reprod-dev","journal_tier":"standard","journal_if":1.4,"is_mock":false,"summary_zh":"研究在日本黑牛中评估一种简化短程激素方案：采卵前5天用孕酮释放装置（CIDR）联合苯甲酸雌二醇同步卵泡波，采卵前2天单次注射FSH，以改善卵母细胞质量及IVP/ET结局。共12头牛，处理组与未处理组各6头。处理组优质卵母细胞比例（63.0% vs 47.5%）、每次OPU可移植囊胚数（4.0 vs 2.6）及IVP囊胚移植后妊娠率（55.6% vs 19.0%）均显著更高（P<0.05）。结论提示该仅需两次操作的方案可改善日本黑牛OPU-IVP与ET结局。","summary":"We evaluated a simple, short hormonal protocol comprising follicular wave synchronization using a progesterone-releasing device and estradiol benzoate 5 days before ovum pick-up (OPU) followed by a single follicle-stimulating hormone injection 2 days before OPU to improve oocyte quality and outcomes of in vitro embryo production (IVP) and embryo transfer (ET) in Japanese Black cows. A total of 12 cows were used in this study, with 6 cows in each of the Treatment and Non-Treatment groups. The proportion of good-quality oocytes, transferable blastocysts per OPU session, and pregnancy rate following ET of IVP-derived blastocysts were all higher in the Treatment group than in the Non-Treatment group (63.0 ± 7.7% vs. 47.5 ± 7.6%, 4.0 ± 0.7 vs. 2.6 ± 0.5, and 55.6% vs. 19.0%, respectively; P < 0.05). These results demonstrate that the present protocol, which requires only two procedures before OPU, effectively improves OPU-IVP and ET outcomes in Japanese Black cows.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}