{"id":112,"title":"CircRNA SNX11 promotes endometrial cell proliferation through GPER1 by sponging hsa-miR-1343-3p","title_zh":"环状RNA SNX11通过海绵吸附hsa-miR-1343-3p上调GPER1促进子宫内膜细胞增殖","url":"https://pubmed.ncbi.nlm.nih.gov/42971168","doi":"10.5468/ogs.26097","pmid":"42971168","journal":"Obstet Gynecol Sci","kind":"paper","lang":"en","published_at":"2026-11-02T00:00:00+00:00","fetched_at":"2026-11-08T03:43:53+00:00","authors":["Shi Y","Liang X","Li M","Peng J","Chang Y","Fang C","Zhang M"],"primary_topic":"endometrium","topics":[{"slug":"endometrium","score":5.0,"hits":["endometrial","endometrial receptivity"]},{"slug":"art-clinical","score":4.0,"hits":["implantation failure","in vitro fertilization","infertility","ivf"]},{"slug":"ethics-policy","score":1.0,"hits":["regulatory"]},{"slug":"omics-methods","score":1.0,"hits":["rna sequencing"]}],"topic_score":5.0,"preference_score":0.0,"preference_labels":[],"llm_score":76.0,"final_score":76.0,"score_dims":{"novelty":72,"rigor":70,"impact":74,"relevance":92,"translation":68},"recommend_reason":"围绕反复种植失败这一临床难点，给出circRNA–miRNA–GPER1的完整ceRNA调控轴并有多重实验验证，机制链条清晰、与内膜容受性直接相关，值得关注；但样本量与体内功能挽救实验信息有限，结论仍属体外层面。","keywords":["反复种植失败","子宫内膜容受性","环状RNA SNX11","GPER1","ceRNA","人子宫内膜基质细胞"],"selected":1,"story_id":208,"llm_provider":"deepseek","llm_model":"deepseek-flash","llm_effort":"low","source_key":"pubmed-art","source_name":"PubMed · 辅助生殖临床","source_tier":1,"mention_count":2,"has_interpretation":false,"journal_key":null,"journal_tier":"unknown","journal_if":null,"is_mock":false,"summary_zh":"研究针对反复种植失败（RIF）中子宫内膜细胞增殖下降、容受性不良的问题，通过对子宫内膜组织RNA测序筛选RIF相关核心基因，并在人子宫内膜基质细胞（hESCs）中用miRNA pulldown与双荧光素酶报告基因实验验证调控关系，以CCK-8检测增殖。结果显示GPER1在RIF患者内膜组织中显著下调；circRNA SNX11可海绵吸附hsa-miR-1343-3p从而上调GPER1，促进hESCs增殖、减少凋亡并调控RIF相关通路。作者据此提出circRNA SNX11–miR-1343-3p–GPER1轴可作为改善内膜容受性、克服RIF的潜在治疗靶点。","summary":"OBJECTIVE: Approximately 15% of couples of reproductive age experience infertility. In vitro fertilization (IVF) is the most useful treatment for patients with infertility. However, repeated implantation failure (RIF) is one of the most difficult challenges for the success of IVF, caused by poor endometrial receptivity due to decreased endometrial cell proliferation. This study aimed to investigate the mechanism regulating endometrial cell proliferation to overcome RIF. METHODS: Hub genes involved in RIF were identified by RNA sequencing for endometrial tissues. The regulatory effects of circular RNA (circRNA) and microRNA (miRNA) in human endometrial stromal cells (hESCs) were determined using miRNA pulldown and dual-luciferase reporter gene assays. Cell proliferation was detected using a Cell Counting Kit-8 assay. RESULTS: G protein-coupled estrogen receptor 1 (GPER1) expression was dramatically downregulated in endometrial tissues obtained from patients with RIF, suggesting that GPER1 might be a hub gene involved in RIF. Additionally, circRNA SNX11 increases GPER1 expression via hsa-miR-1343-3p in hESCs. Additionally, circRNA SNX11 promotes cell proliferation, reduces apoptosis, and modulates RIF-related pathways through GPER1 by sponging hsa-miR-1343-3p in hESCs. CONCLUSION: This study reveals that circRNA SNX11 facilitates endometrial cell proliferation by upregulating GPER1 by sponging hsa-miR-1343-3p, providing novel therapeutic targets to overcome RIF.","interpretation_basis":null,"fulltext_status":null,"interpretation":null,"fulltext":{"available":false,"status":null,"reason":"尚未抓取全文（未入选、分数未达阈值，或还没跑 fulltext 步骤）","pmcid":null,"license":null,"chars":0,"source":null,"fetched_at":null}}